摘要
目的研究地木耳多糖的抗氧化性与抑菌活性。方法利用超声波提取法从地木耳中提取多糖,经初步纯化后,对多糖的抗氧化性和抑菌作用进行分析。结果地木耳多糖具有一定的抗氧化能力,且其抗氧化能力在一定的浓度范围内呈现良好的量效关系。地木耳多糖对·OH的清除率最高,最高可达70.53%,对DPPH的清除率次之,最高可达67.72%,对O_2-的清除能力最低,最高可达32.31%。地木耳多糖对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、啤酒酵母均有一定的抑制作用,其中对大肠杆菌的抑菌效果最好,最小抑菌质量浓度为31.25μg/mL;对丹参枯萎病菌无抑制作用。结论地木耳多糖具有一定的抗氧化能力和抗菌能力。
Objective To research the antioxidant and antibacterial activity of polysaccharides from Nostoc commune. Methods Polysaccharide was extracted from Nostoc commune by ultrasonic extraction. After initial purification, the antioxidant and antibacterial effects of polysaccharides were analyzed. Results The polysaccharide from Nostoc commune had certain antioxidant capacity, and its antioxidant capacity showed a good dose-effect relationship within a certain concentration range. The polysaccharide had the highest clearance rate of ·OH, up to 70.53%, followed by DPPH, up to 67.72%, and the lowest removal rate of O2-, up to 32.31%. The Nostoc communepolysaccharide had a certain inhibitory effect on Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Saccharomyces cerevisiae, among which the antibacterial effect on E. coli was the best, the minimum inhibitory mass concentration was 31.25 μg/mL, and it had no inhibitory effect on Salvia miltiorrhiza wilt. ConclusionPolysaccharides from Nostoc Commune has certain anti-oxidation ability and antibacterial ability.
作者
刘丹丹
朱志学
马健
邵波
徐希柱
刘兆玺
李艳玲
LIU Dan-Dan;ZHU Zhi-Xue;MA Jian;SHAO Bo;XU Xi-Zhu;LIU Zhao-Xi;LI Yan-Ling(College of Life Sciences,Taishan Medical University,Taian 271016,China)
出处
《食品安全质量检测学报》
CAS
2019年第4期921-926,共6页
Journal of Food Safety and Quality
基金
山东省重点研发计划项目(2016GSF202002)
国家大学生创新创业训练计划项目(201610439261)
国家自然科学基金项目(31500148)~~
关键词
地木耳
多糖
抗氧化性
抑菌作用
Nostoc commune
polysaccharide
antioxidation
bacteriostasis
