摘要
目的研究桑枝降血糖的活性部位,对桑枝进行提取分离,初步得出降糖活性成分。方法桑枝渗漉后经过不同溶剂萃取,通过小鼠体内降糖实验,以高糖小鼠的血糖下降百分率为指标,确定桑枝的降糖活性萃取部位。采用聚酰胺树脂对活性萃取部位进行分离,同样通过降糖实验,以高糖小鼠的血糖下降百分率为指标,确定桑枝的降糖活性分离部位。采用理化显色反应,初步鉴别降糖活性物质。结果通过对桑枝提取液萃取后,降糖效果最明显的为正丁醇萃取部位,高糖小鼠血糖下降百分率为26.20%。正丁醇萃取部位采用聚酰胺树脂分离后降糖效果最明显的为10%乙醇洗脱部位,血糖下降百分率为31%。理化显色反应结果表明活性部位中含有生物碱类物质。结论初步确定桑枝中降血糖活性部位中存在生物碱类成分。
Objective To study the active part of Ramulus Mori that reduces blood glucose,so as to extract it and obtain the active parts. Methods The percentage of reduction blood glucose was taken as index and the in vivo hypoglycemic experiment was used to optimize the active components which can lower the blood glucose percolated from Ramulus Mori extracting solution. Polyamide was used to separate the active extraction,and the percentage of reduction blood glucose was also taken as index and the same experiment was used to optimize the active separated components which could lower the blood. Chromogenic reaction was used to identify the active extraction preliminarily. Results The experiment proved that the most effective extraction was Ramulus Mori extracted by n- butyl alcohol and the percentage of reducing blood glucose was26. 20%. The active extraction was separated by polyamide used different concentration of ethanol,then the most effective part was eluted by 10% ethanol and the percentage of reducing blood glucose was 31%. The result of chromogenic reaction proved that alkaloid compounds and amino acids were existed in the effective part. Conclusion It is initially identified that alkaloid compounds exist in active sites of reducing blood glucose in Ramulus Mori.
出处
《世界中西医结合杂志》
2015年第9期1219-1221,共3页
World Journal of Integrated Traditional and Western Medicine
关键词
桑枝
生物碱
降血糖
Ramulus Mori
Alkaloid compounds
Reducing blood glucose
