摘要
为探讨NKX6-1基因启动子甲基化与HPV16感染在新疆维吾尔族妇女宫颈癌发生中的作用。收集43例新疆维吾尔族妇女正常宫颈组织、30例宫颈上皮内瘤样病变(CIN)和48例宫颈癌组织。采用PCR方法检测HPV16感染;甲基化特异性PCR方法检测NKX6-1基因启动子区甲基化状况,并分析启动子甲基化与HPV16感染与宫颈癌的相关性。结果显示,正常宫颈组,CIN和宫颈癌组中的NKX6-1基因甲基化率分别为11.63%、46.67%和77.08%,差异有统计学意义(P<0.01);HPV16的感染率分别为13.95%,36.67%和66.66%,三组差异均有统计学意义(P<0.01)。NKX6-1甲基化与HPV16感染无显著相关性(P>0.05)。由此可知,NKX6-1基因启动子甲基化与HPV16感染无相关性,但与维吾尔族宫颈癌发生相关。
Objective to explore the NKX6- 1 gene promoter methylation in cervical cancer of tumorigenesis in Uyghur women.Methods The promoter methylation of NKX6- 1 was investigated by methylation- specific polymerase chain reaction(MSP) in 48 cervical cancer tissues, 30 cervical intraepithelial neoplasia(CIN) tissues and 43 normal cervical tissues. HPV16 infection detected by PCR method. The correlation to clinical stage and HPV16 infection of cervical cancer was analyzed. Results The methylation frequency of NKX6- 1 promoter from normal cervical tissues to cervical cancer tissues was 11.63%, 46.67% and77.08%, respectively; The infection frequency of HPV16 was 13.95%, 36.67% and 66.66%, respectively, and the difference is statistically significant(P<0.01). The correlation between methylation ratio of NKX6- 1 and infection ratio of HPV16 has nostatistically significance(P>0.05). Conclusion NKX6- 1 gene prom oter methylation is correlated with the cervical cancer tumorigenesis of Uyghur women, while no correlation with HPV16.
出处
《石河子大学学报（自然科学版）》
CAS
2015年第3期310-314,共5页
Journal of Shihezi University(Natural Science)
基金
国家科技支撑计划项目(2013BAI05B0503)
国家自然科学基金项目(30860302
30660193)
国际合作与交流专题项目(2010DFB34100)
新疆兵团国际科技合作项目(2013BC003)
石河子大学重大科技攻关计划项目(GXJS2013-ZDGG05)
