摘要
目的探讨板蓝根提取物对放射性肺损伤大鼠肺组织TGF-β表达的影响。方法将72只健康成年清洁级雌性Wistar大鼠随机分为空白对照组(A组)24只,单纯照射组(B组)24只,照射加中药组(C组)24只,A、B 2组以蒸馏水2 mL/200 g灌胃1周后,C组以每天2 mL/200g(1.6 g/200 g)灌胃服用板蓝根提取物1周,开始照射,照射期间,每周测体质量1次,至照射结束。分别在完成10次照射后(第30天),以及在照射开始后的第60天、120天,分别检测3组大鼠肺组织中TGF-β的表达;另外对各组大鼠进行肺组织形态学观察。结果 B组大鼠TGF-β的表达于照射第30天时达到高峰;A、C组大鼠TGF-β表达在各时间点均低于B组(P<0.01)。空白对照组A组大鼠肺组织未见明显改变。B组大鼠肺间质肺泡腔内观察到炎性细胞,肺泡腔结构破坏、肺间质水肿,肺泡腔及肺间质内炎性出血;C组大鼠肺间质炎性细胞较少,肺泡腔破坏不明显,无肺间质水肿,也无炎性出血症状发生,B、C 2组间比较不明显。结论板蓝根提取物可减少放射性损伤大鼠肺成纤维细胞TGF-β表达,可减少放射性肺损伤的功效。具有保护肺组织不受损害,抑制肺泡通透性,预防肺水肿发生的作用。
Objective To investigate the effect of extraction of radix isatidis on the expression of transforming growth factor(TGF-β)in lung tissues of rats with radioactive lung injury.Methods 72 female Wistar rats were randomly divided into 3 groups,blank control group(groupA,n=24),simple irradiation group(group B,n=24),irradiation with Chinese medicine group(group C,n=24).Rats in group A and group B were given distilled water 2 mL/200g by gavage for one week,the rats in group C were given extraction of radix isatidis 2 mL/200g(1.6 g/200 g)per day for one week,then the rats received irradiation,during irradiation,the rats'' weight was measured once a week,to illuminate the end.After 10-time irradiation(the 30th day), and at 60 days and at 120 days after irradiation,the expression levels of TGF-βin lung tissue were detected;In addition each rat lung tissue morphology was observed.Results The expression levels of TGF-βprotein in group B reached peak at the 30 days after irradiation,which at different time points in group A and group C was significantly lower than that in group B(P<0.01).Rats of group A had no significant change in lung tissue.Rats of group B were observed inflammatory cells in interstitial pulmonary alveolar,alveolar structural damaging,interstitial pulmonary edema,interstitial hemorrhage and inflammatory pulmonary with alveolar cavity;Rats of group C pulmonary interstitial fewer inflammatory cells in alveolar damage were not obvious, with no-pulmonary interstitial edema, hemorrhage and no inflammatory symptoms, comparison between group B and C was not obvious. Conclusion Extraction of radix isatidis could reduce the expression levels of transforming growth factor(TGF-β)in lung tissues of rats with radioactive lung injury ,has better preventive effect on radioactive lung injury,could protect lungtissue from damaging,inhibite alveolar permeability,and present the effect of pulmonary edema.
出处
《中国生化药物杂志》
CAS
北大核心
2014年第7期37-39,共3页
Chinese Journal of Biochemical Pharmaceutics
基金
山东省自然科学基金(Y2008C123)
关键词
板蓝根提取物
放射性肺损伤
转化生长因子-Β
extraction of radix isatidis
radioactive lung injury
transforming growth factor-β
