摘要
用17ɑ-甲睾酮混合饲料投喂诱导2批虹鳟Oncorhynchus mykiss伪雄鱼(三、四龄,平均体质量分别为2 200 g和3 056 g),以正常二倍体群体为对照(三、四龄平均体质量2 510 g和3 600 g),采用组织学和放射性免疫(RIA)方法,每2个月采血一次研究伪雄鱼和正常二倍体血清中性类固醇激素雌二醇(E2)和睾酮(T)的周年变化。结果表明,正常虹鳟和伪雄鱼精巢的组织细胞结构没有明显差异,伪雄鱼性腺组织形态与卵巢相似。3龄和4龄伪雄鱼血清中E2含量均在12月达到峰值,而3龄鱼的T浓度在12月达到峰值,4龄则在翌年2月达到峰值;3龄雌鱼血清中E2含量在10月达到峰值,4龄则在8月达到峰值,而T浓度均在12月达到峰值,进入繁殖期后E2含量开始下降;3龄和4龄正常雄鱼血清T浓度均在12月达最大值,E2在10月达峰值。结果表明,测定血清性类固醇激素浓度可用于准确判断鱼类的生殖状态,为正确利用虹鳟伪雄鱼提供参考。
The annual fluctuations in serum estradiol(E2)and testosterone(T)were comparatively determined in 3 and 4 years old male-induced with body weight of 2 200 g and 3 056 g by dietary 17-methyl testosterone and normal diploid rainbow trout(Oncorhynchus mykiss)with body weight of 2 510 gand 3 600 g by a histological method and radio immunosorbent assay(RIA). The results showed that there were no significant differences in cell structures in normal male and male-induced rainbow trout gonads, and the gonadal morphology of male-induced rainbow trout was the similar with that of the normal females. The maximal serum E2 and T levels were observed in 3 and 4 years old male-induced rainbow trout in December each year, and in December and February of next year, respectively. The serum E2 level was peaked in 3 years old normal females in October, and the maximum in 4 years old female in August. The maximal serum T levels were found in 3 and 4 normal females in December, and the E2 level was found to begin to decline in the breeding period. The maximal serum T levels were all found in 3 and 4 years old normal male rainbow trout in December,and E levels all peaked in October. The findings indicated that the measurement of serum sex steroid hormone level could determine exactly the reproduction conditions of fish, which would provide important technologic basis with reasonable utlization of male-induced rainbow trout.
作者
谷伟
徐革锋
户国
黄天晴
张玉勇
白庆利
王炳谦
GU Wei;XU Ge-feng;HU Guo;HUANG Tian-qing;ZHANG Yu-yong;BAI Qing-li;WANG Bing-qian(Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China)
出处
《水产学杂志》
CAS
北大核心
2019年第1期7-11,共5页
Chinese Journal of Fisheries
基金
中央级公益性科研院所基本科研业务费专项(HSY201404
HSY201601)
特色淡水鱼产业技术体系(CARS-46)
关键词
虹鳟
伪雄鱼
血清
激素
rainbow trout
male-induced fish
serum
sex steroid hormone
