摘要
以鱼油为脂肪源,分别配制脂肪水平为3.7%、6.8%、9.6%、13.1%、15.6%的5种实验饲料,对平均初始体质量为(7.13±0.03)g的许氏平鲉进行为期60 d的饲养实验,探讨饲料脂肪水平对许氏平鲉形体指数、脂肪沉积、血液生化指标与脂肪代谢酶活性的影响。结果表明:肝体指数、脏体指数随着饲料脂肪水平的升高显著提高(P<0.05),脂肪水平对肥满度没有显著影响(P>0.05);许氏平鲉鱼体脂肪沉积随饲料脂肪水平的增加显著提高(P<0.05),15.6%全鱼、肌肉、肝脏脂肪含量最高,显著高于3.7%、6.8%组(P<0.05)。随着脂肪添加量的增加,许氏平鲉肝脏葡萄糖-6-磷酸脱氢酶(G6PDH)和苹果酸脱氢酶(MHD)活性显著降低(P<0.05),15.6%与13.1%组G6PDH、MHD活性显著低于3.7%和6.8%组(P<0.05);肝酯酶(HL)和脂蛋白酯酶(LPL)活性显著升高(P<0.05),15.6%与13.1%组HL和LPL活性显著高于3.7%和6.8%组(P<0.05)。血液甘油三酯(TG)和总胆固醇(CHO)含量随着饲料脂肪水平增加有下降的趋势,15.6%组的TG和CHO含量显著低于3.7%和6.8%组(P<0.05);血液中高密度脂蛋白胆固醇(HDLC)、谷草转氨酶(AST)、谷丙转氨酶(ALT)与碱性磷酸酶(ALP)活性呈上升趋势(P<0.05),15.6%组最高;低密度脂蛋白胆固醇(LDLC)呈先上升后下降的趋势(P<0.05)。研究表明,饲料脂肪水平对许氏平鲉形体指数、脂肪沉积、血液生化指标及脂肪代谢酶活性影响显著,但过高的脂肪水平将使许氏平鲉肝脏脂肪沉积增多,当饲料脂肪水平高于9.6%时不利于鱼类健康生长。
We evaluated the effects of dietary lipid levels on body index,lipid deposition,blood biochemistry indices and lipid metabolic enzyme activities of Sebastes schlegeli.Juvenile fish[average initial body weight:(7.13 ±0.03) g]were randomly divided into 5 groups and fed diets containing different lipid levels(3.7%,6.8%,9.6%,13.1% and 15.6%) using fish oil as the lipid source for 60 d.The results showed that the hepatosomatic index(HSI) and viscerosomatic index(VSI) of S.schlegeli increased with increasing dietary lipid levels(P < 0.05),while the condition factor(CF) had no significant differences among groups(P <0.05).The crude lipid content of whole body,muscle and liver of S.schlegeli increased with increasing dietary lipid levels(P < 0.05).The crude lipid contents of whole body,muscle and liver in the group fed15.6% lipid were significantly higher than those of the groups fed 3.7% and 6.8% lipid(P < 0.05).Dietary lipid had a significant effect on lipid metabolic enzymes(P < 0.05).The activities of hepatic glucose-6-phosphate dehydrogenase(G6PDH) and malic dehydrogenase(MDA) decreased with increasing lipid level,but the activities of lipoprotein lipase(HPL) and hepaticlipase(HL) increased(P < 0.05).The activities of G6 PDH and MDA were significantly lower in the groups fed 15.6% and 13.1% lipid than those of the groups fed 3.7% and 6.8% lipid(P < 0.05),but the activities of HPL and HL were significantly higher(P < 0.05).The contents of blood triglyceride(TG) and cholesterol(CHO) decreased with increasing dietary lipid levels,but the contents of blood high density lipoprotein cholesterol(HDL-C),glutamic oxaloacetic transaminase(AST) and glutamic pyruvic transaminase(ALT) increased(P < 0.05).The contents of blood TG and CHO in the group fed 15.6% lipid were significantly lower than those of the groups fed3.7% and 6.8% lipid(P < 0.05),but the contents of blood HDL-C,AST and ALT were highest.The contents of blood low density lipoprotein cholesterol(LDL-C) initially increased with increasing lipid levels,but then decr
出处
《水产学报》
CAS
CSCD
北大核心
2014年第11期1879-1888,共10页
Journal of Fisheries of China
基金
国家海洋局公益性行业科研专项(201205025)
关键词
许氏平鲉
脂肪水平
形体指数
脂肪沉积
血液生化指标
脂肪代谢酶
Sebastes schlegeli
dietary lipid level
body index
lipid deposition
blood biochemistry indices
lipid metabolic enzyme
