摘要
目的:探讨DAPK、c-mcy、P53、Caspase-3在子宫内膜癌中的表达及临床意义。方法:筛选2015年1月至2016年12月我院妇科收治的121例子宫内膜癌、68例子宫内膜非典型增生以及40例正常子宫内膜患者作为观察对象。对采集到的标本进行相应处理,并应用相应的试剂盒进行免疫组化检测,观察各组标本的免疫组化结果,对比不同组别病变中DAPK、c-mcy、P53、Caspase-3的表达效果。结果:DAPK启动子甲基化率、c-mcy、、P53蛋白检测结果显示子宫内膜癌P53阳性率(P<0.05)明显高于非典型增生组与正常内膜组;Caspase-3在三组标本中表达效果存在显著差异,以正常内膜组表达效果最高,而子宫内膜癌组表达效果最低,不同组别的c-mcy检测阳性率比较存在显著差异,P<0.05,差异具有统计学意义。结论:DAPK、c-mcy、P53、Caspase-3的异常表达与子宫内膜癌的发生发展密切相关,其可能与细胞增殖、凋亡功能的异常有关,通过对DAPK、c-mcy、P53、Caspase-3表达的检测可协助判断患者的临床预后,并对疾病的早期诊断与治疗具有重要的指导作用。
Objective: To investigate the expression and clinical significance of DAPK, c-mcy, P53 and Caspase-3 in endometrial carcinoma. Methods: From January 2015 to December 2016, 121 cases of endometrial carcinoma, 68 cases of atypical hyperplasia of endometrium and 40 cases of normal endometrial patients were observed. The expression of DAPK, c-mcy, P53 and Caspase-3 in different groups were observed by immunohistochemistry and the expression of DAPK, c-mcy, P53 and Caspase-3 in different groups were observed by immunohistochemistry. effect. Results: The methylation rate of DAPK promoter, c-mcy and P53 protein showed that the positive rate of P53 in endometrial carcinoma(P <0.05) was significantly higher than that in atypical hyperplasia group and normal endometrium group. Caspase-3 group The expression of c-mcy in different groups was significantly different(P <0.05), and the difference was statistically significant(P <0.05). The expression of c-mcy was significantly higher than that of normal endometrium(P <0.05), and the difference was statistically significant significance. Conclusion: The abnormal expression of DAPK, c-mcy, P53 and Caspase-3 is closely related to the development of endometrial carcinoma. It may be related to cell proliferation and apoptosis abnormality. DAPK, c-mcy, P53, The detection of Caspase-3 expression can help to judge the clinical prognosis of patients, and early diagnosis and treatment of disease has an important guiding role.
出处
《中国妇幼健康研究》
2017年第S2期117-117,共1页
Chinese Journal of Woman and Child Health Research
