摘要
目的 :分析针对分子量为 38kD和 或 15 16 5kD多肽抗原的抗核糖体抗体 (anti ribosomalantibodies)与SLE的关系 ,探讨抗核糖体抗体的阳性判断标准及其在SLE中的检出情况。方法 :收集病人血清 2 6 2例 ,包括SLE115例、RA5 7例、硬皮病 2 0例、MCTD39例及其他免疫性疾病 31例 ,用免疫印迹法 (Western blot)检测其抗ENA抗体谱。结果 :分别以同时出现 38kD和 16 5 15kD蛋白条带以及出现 38kD蛋白条带为判断抗核糖体抗体阳性的标准 ,则该抗体对诊断SLE的敏感性和特异性结果分别为 2 8 7% (33 115 )、96 6 % (14 2 14 7)及 17 4 % (2 0 115 )、97 3% (14 3 14 7) ,两者比较敏感性有显著性差异 (P <0 0 5 ) ,特异性无显著性差异 (P >0 0 5 ) ;仅 38kD分子阳性的 14例样本中 ,SLE占大多数 ,显著多于其它疾病 (71 4 %比 2 8 6 % ,P <0 0 5 ) ;38kD分子阳性同时伴抗Sm抗体阳性者多于抗Sm抗体阴性者 ,但无显著性差异 (6 4 3%比 35 7% ,P >0 0 5 )。结论 :抗核糖体抗体 38kD分子与SLE密切相关 ,而与抗Sm抗体相关性不强。以 38kD为主要抗原多肽判断抗核糖体抗体 ,不仅可以提高该抗体的阳性检出率 ,同时也不会降低其对SLE诊断的特异性 ,该判断方法值得推广应用。
Objective:Analysis the relationship between antiribosomal antibodies with molecular weight 38 kD and/or 15/16.5 kD protein and SLE,then to discuss the positive standard of anti ribosomal antibodies and its prevalent rate in SLE patients.Methods:262 sera from 115 SLE, 57 RA,20 SSc,39 MCTD and 31 other immune diseases were detected anti ENA antibodies by Western blot method.Results:Respectively, use 38 kD and 15/16.5 kD protein bands appear simultaneously and 38 kD protein bands appear as anti ribosomal antibodies’ positive standard, the sensitivity and specificity of anti ribosomal antibodies to SLE were 17.4%,97.3% and 28.7%,96.6% respectively.The sensitivity had significant difference (P<0 05) but the specificity had no (P>0 05).SLE patients were dramatically dominated in 14 anti ribosomal 38 kD antibody positive patients(71.4% vs. 28.6%,P<0 05).Anti ribosomal 38 kD positive patients with anti Sm positive were more than those with anti Sm negative but have no significant difference(64.3% vs. 35.7%,P>0 05).Conclusion:Anti ribosomal 38 kD antibody was closely associated with SLE but not with anti Sm antibody. Use 38 kD protein as the primary standard to verdict anti ribosomal antibody,not only can improve the positive rate of anti ribosomal antibody, but also do not depress its diagnostic specificity for SLE.So it’s worthy to spread this method. [
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2003年第9期638-640,共3页
Chinese Journal of Immunology
