摘要
目的观察糖尿病大鼠下丘脑室旁核(PVN)内神经元型一氧化氮合酶(nNOS)免疫阳性神经元数目的变化,探讨PVN内NO在糖尿病发展中的作用机制。方法建立链脲佐菌素诱导的糖尿病大鼠模型,于第2、7、12周末取脑组织进行ABC免疫细胞化学法染色,定量分析PVN内nNOS免疫阳性神经元数目变化。结果2周时糖尿病组大鼠PVN内nNOS免疫阳性神经元数目与对照组相比无显著差异(P>0.05);7、12周时糖尿病组大鼠PVN内nNOS免疫阳性神经元数目显著高于对照组(P<0.05)。结论PVN内nNOS免疫阳性神经元数目在糖尿病中、后期明显升高,糖尿病状态下PVN内nNOS活性改变可能与糖尿病神经内分泌及肾上腺素能途径改变有关。
Objective To explore the role of nitric oxide in the development of diabetes mellitus by observing the changes of neuronal nitric oxide synthase (nNOS)-immunoreactive (nNOS-ir) neurons in the paraventricular hypothalamic nucleus (PVN) of diabetic rats. Methods Diabetic rat models were established by means of intraperitoneal streptozotocin injections. At the end of 2, 7 and 12 weeks after model establishment, tissue sampling was performed and the number of nNOS-ir neurons in PVN were counted and quantitatively analyzed. Results The number of nNOS-ir neurons in PVN of diabetic rats remained normal 2 weeks after model establishment (P>0.05) but was significantly increased by the time of 7 weeks (P<0.05); at the 12th weeks, the number of nNOS-ir neurons of diabetic group was still greater than that of the control group (P<0.05). Con-clusion The number of nNOS-ir neurons in PVN increases significantly in middle and advanced stages of diabetes, suggesting the possible relations between the nNOS activity changes in PVN and the changes in cerebral neuroendocrine and adrenal ac-tivity in diabetic condition.
出处
《第一军医大学学报》
CAS
CSCD
北大核心
2003年第7期692-695,共4页
Journal of First Military Medical University
基金
广东省卫生厅课题(A1998290)
广东省中医药管理局课题(100068)~~
关键词
糖尿病
大鼠
下丘脑室旁核
内神经元型一氧化氮合酶
免疫阳性神经元
diabetes mellitus/immunology
paraventricular hypothalamic nucleus
neurons
nitric oxide synthase
nitric oxide
