摘要
[目的]探讨人参皂甙对急性肾衰大鼠的肾保护作用与下丘脑室旁核ChAT免疫反应活性(ChAT-IR)变化的相关性。[方法]选用健康雄性SD大鼠,随机分为4组:ARF+NS组、ARF+GS组、NS+NS组和NS+GS组。采用甘油致急性肾衰的动物模型,通过整体实验和免疫组织化学的方法,观察急性肾衰大鼠口服人参皂甙(25 mg/2mL)48 h后,血清尿素氮(blood urea nitrogen,BUN)和肌酐(creatinine,Cre)水平的变化,肾皮质匀浆丙二醛(malon-dialdehyde,MDA)和还原型谷胱甘肽(reduced glutathione,GSH)水平的变化,同时观察下丘脑室旁核ChAT免疫反应活性的变化。[结果]急性肾衰大鼠口服生理盐水2 mL处置48 h(ARF+NS组)后,血清尿素氮和肌酐水平显著升高;明显高于NS+NS组(P<0.05)。而急性肾衰大鼠口服人参皂甙处置48 h(ARF+GS组)后,血清尿素氮和肌酐水平降低,明显低于ARF+NS组(P<0.05),但与NS+NS组差异无显著性意义。ARF+NS组肾皮质匀浆MDA水平明显升高(P<0.05),GSH水平明显降低(P<0.05);而ARF+GS组肾皮质匀浆MDA水平降低(P<0.05);GSH水平升高(P<0.05)。免疫组织化学的结果显示ARF+NS组下丘脑室旁核ChAT-IR明显增强(P<0.05)。ARF+GS组下丘脑室旁核ChAT-IR进一步增加,明显高于ARF+NS组(P<0.05)。[结论]急性肾衰大鼠口服人参皂甙48 h后,能显著改善肾衰大鼠的肾功能,明显减轻肾组织氧化损伤,并增强肾组织抗氧化损伤的能力,表明口服人参皂甙具有抗急性肾衰和肾保护的作用。下丘脑室旁核胆碱能神经元活性的增强可能是人参皂甙抗急性肾衰和肾保护的作用的中枢机制之一。
[Objective] To investigate the protective effect of ginsenoside on acute renal failure（ARF） rats and the correlation between the effect and changes of ChAT-immunoreactivity in hypothalamic paraventricular nucleus.[Methods] Male SD rats were randomly divided into four groups： ARF＋NS group,ARF＋GS group,NS＋NS group and NS＋GS group.Glycerol-induced acute renal failure in rats was employed.Blood urea nitrogen（BUN） and Creatinine（Cre） were measured by commercial regents,as well as malondialdehyde（MDA）,reduced glutathione（GSH） in renal cortex homogenate by experiment in vivo.Meanwhile,the changes of ChAT-IR in the PVN were observed by immunohistochemistry.[Results] Glycerol-induced ARF rats treated with NS（2 mL） for 48 h showed a significant increase in BUN and Cre（P〈0.05）,compared with NS＋NS group.However,Glycerol-induced ARF rats treated with GS for 48 h showed a significant decrease in BUN and Cre（P〈0.05）,compared with ARF＋NS group.In ARF＋NS group,the level of MDA in renal cortex homogenate significantly increased（P〈0.05）,but GSH markedly decreased（P〈0.05）.However,in ARF＋GS group the level of MDA in renal cortex homogenate significantly decreased,but GSH markedly increased（P〈0.05）.Immunohistochemistry showed an obvious increase of ChAT-IR in the PVN in ARF＋NS group（P〈0.05）,compared with that in NS＋NS group;but ChAT-IR was further enhanced in ARF＋GS group（P〈0.05）,compared with that in ARF＋NS group.[Conclusion] Our results indicated that ginsenoside administrated orally in glycerol-induced ARF rats significantly improved renal function,increased antioxidative effects.These findings suggested that ginsenoside may have a strong renal protecting effect against glycerol-induced acute renal failure.Our results also indicated that ginsenoside administrated orally could further increase the expression of ChAT-IR in the PVN in ARF rats.Consequently,we provided a new evidence that brain cholinergic neurons in the PVN contributed to the r
出处
《大连医科大学学报》
CAS
2008年第5期431-434,438,共5页
Journal of Dalian Medical University
