摘要
目的：探讨人参茎叶皂甙对Ｄ－半乳糖拟衰老大鼠的作用。方法：Ｗｉｓｔａｒ雌性大鼠自５０日龄起每日皮下注射Ｄ－半乳糖４８ｍｇ／ｋｇ，连续６周。其中一组同时喂饲人参茎叶皂甙（２５ｍｇ／２０ｇ饲料比例掺入）。应用邻苯三酚法、ＴＢＡ比色法、放射免疫分析法分别测定下丘脑超氧化物歧化酶（ＳＯＤ）活性、脑组织丙二醛（ＭＤＡ）含量、血清及原代垂体细胞培养液中催乳素（ＰＲＬ）浓度。结果：人参茎叶皂甙可阻止Ｄ－半乳糖诱发下丘脑ＳＯＤ活性下降、脑组织ＭＤＡ含量增加、血清ＰＲＬ升高以及垂体细胞对促甲状腺素释放激素（ＴＲＨ）、血管活性肠肽（ＶＩＰ）及多巴胺（ＤＡ）刺激的反应性降低等作用。结论：人参茎叶皂甙有对抗Ｄ－半乳糖致“衰老”
Objective: To further understand the Ginsenoside from stem and leaf(GSL) antiaging effects in Dgalactose induced aging mode. Methods: Wistar female rat of fifty days were subcutenously injected with Dgalactose 48 mg/kg daily for 6 weeks. During this period half of the rats were fed with GSL(25 mg/20 g food). MDA,SOD and PRL were determined by TBA colorimetry,pyrogallol autoxidation and radioimmunoassay respectively. Results: The Dgalactose treated rats showed elevated serum PRL and in vitro studies revealed enhanced basal PRL secretion but significantly attenuated response to VIP,TRH and DA.These changes are similar with those occurred in naturally aging rats and are results of disturbance in neuroendocrine intergration. Daily administration of GSL prevented all these changes just as it did in naturally aging animals. GSL also prevented the decrease of hypothalamic SOD and accumulation of brain MDA. Conclusion: The pressent results also reconfirm the validity of Dgalactose aging model in the scree
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1999年第3期164-165,共2页
Journal of China Medical University
基金
沈阳市科委基金
关键词
衰老
半乳糖
人参茎叶皂甙
下丘脑
实验研究
aging
Dgalactose
Ginsenoside
prolactin
pituitary
hypothalamusning of antiaging medicines.
