摘要
目的:探讨真武汤中不同附子剂量对肾病综合征(Nephrotic syndrome,NS)大鼠疗效的影响,并研究其作用机制。方法:50只大鼠随机分成附子高剂量组、附子中剂量组、附子低剂量组、模型组、空白组,每组10只,除空白组外,其余大鼠按5 mg/kg的剂量一次性尾静脉注射阿霉素溶液构建NS模型,第3周末检测造模是否成功。附子高、中、低剂量组大鼠每天给予含附子生药1.52 g/kg、0.92 g/kg、0.52 g/kg的真武汤灌胃,模型组和空白组予等量生理盐水灌胃,均灌胃4周,第7周末检测各组大鼠的24 h尿蛋白、血清白蛋白(Serum albumin,Alb)、尿素氮(Blood urea nitrogen,BUN)、肌酐(Serum creatinine,SCr)含量。结果:与空白组比较,模型组3周后24 h尿蛋白明显升高(P <0.01);与模型组比较,附子高、中剂量组NS大鼠的精神、活动、毛色、摄食量等状况显著改善,24 h尿蛋白、BUN、SCr明显降低,血清白蛋白升高(P <0.05,P <0.01),附子低剂量组效果不明显。结论:附子高、中、低剂量组均能降低NS大鼠24 h尿蛋白、血清BUN、SCr,升高Alb,且附子高剂量组效果更为显著。
Objective:To discuss the effect of Zhenwu tang in different doses of aconite roots on protein metabolism and clinical effect of rats with nephrotic syndrome(NS),and study its functional mechanism.Methods:50 rats were divided into the high-dose aconite root group,the middle-dose aconite root group,the low-dose aconite root group,the model group and the blank group randomly,10 rats in each group.Except the blank group,the other groups received one single intravenous injection of adriamycin solution in 5 mg/kg via tail vein to establish the model of NS.In the end of the 3 rd week,detected whether the model was successful.Rats in the groups of aconite roots in low,middle and high doses were given Zhenwu tang containing crude aconite roots in 1.52 g/kg,0.92 g/kg and 0.52 g/kg via gavage respectively every day,while the model group and the blank group were given equivalent normal saline via gavage.The gavage in each group lasted for 4 weeks.In the end of the 7 th week,detected the contents of 24-hour urine protein,serum albumin(Alb),blood urea nitrogen(BUN)and serum creatinine(SCr).Results:After 3 weeks,comparing with the blank group,the level of 24-hour urine protein in the model group was significantly increased(P<0.01);comparing with the model group,such conditions as vitality,activities,hair color and food intake of rats in the groups of aconite roots in middle and high doses were significantly improved,the levels of 24-hour urine protein,serum BUN and SCr were decreased,and the level of Alb was increased(P<0.05,P<0.01);there was no significance being found in the decreasing effect in the low-dose aconite root group.Conclusion:The groups of aconite roots in low,middle and high doses can decrease the levels of 24-hour urine protein,serum BUN and SCr and increase the levels of Alb,among which the high-dose aconite root group has more significant clinical effect.
作者
徐辉辉
李索咪
洪俊豪
童培建
XU Huihui;LI Suomi;HONG Junhao;TONG Peijian
出处
《新中医》
CAS
2019年第5期53-55,共3页
New Chinese Medicine
