摘要
为了开发一种新的食品防腐保鲜剂,本文初步研究了3-蒈烯对铜绿假单胞菌的抑菌活性及机理。通过肉汤稀释法测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC),评价了3-蒈烯的抑菌活性,考察了3-蒈烯对细菌生长曲线、细胞形态、细胞膜通透性、膜电位及呼吸链脱氢酶等的影响。结果表明:3-蒈烯对铜绿假单胞菌的MIC为20mL/L,MBC为40mL/L。3-蒈烯可以抑制铜绿假单胞菌的生长,破坏细胞的正常形态,提高细胞膜的通透性,造成蛋白质、电解质的外泄。3-蒈烯能够降低铜绿假单胞菌菌体的膜电位,干扰细胞正常代谢活动,还能抑制细胞内呼吸链脱氢酶的活性。综上所述,3-蒈烯通过破坏铜绿假单胞菌细胞膜,抑制细菌正常生长,导致细菌细胞死亡。
In order to develop natural food preservatives, the primary antibacterial activity and mechanism of 3-carene against Pseudomonas aeruginosa were studied. The antibacterial activity of 3-carene was evaluated based on the minimum inhibitory concentration(MIC) and minimum bactericide concentration(MBC) by broth dilution method. The effect of 3-carene was investigated by bacterial growth curve, scanning electron microscopy, electric conductivity, membrane potential and respiratory chain dehydrogenase. The results showed that: the MIC and MBC of 3-carene against Pseudomonas aeruginosa were 20 mL/L and 40 mL/L, respectively. 3-carene could inhibit the growth of Pseudomonas aeruginosa, damage the normal cell morphology, improve the permeability of cell membrane, which cause the leakage of proteins and electrolytes. Moreover, 3-carene reduced the membrane potential, interfered with normal metabolic activity, as well as inhibited the activity of respiratory chain dehydrogenase in cells. Studies have shown that 3-carene inhibited the normal growth of Pseudomonas aeruginosa, leading to bacterial cell death by the destruction of cell membrane.
作者
刘雪
何英兰
胡月英
陈卫军
陈海明
钟秋平
陈文学
LIU Xue;HE Yinglan;HU Yueying;CHEN Weijun;CHEN Haiming;ZHONG Qiuping;CHEN Wenxue(College of Food Science and Technology,Hainan University,Haikou,Hainan 570228,China;Chunguang Agro-Product Processing Institute,Wenchang,Hainan 571333,China;College of Materials and Chemical Engineering,Hainan University,Haikou,Hainan 570228,China)
出处
《热带作物学报》
CSCD
北大核心
2019年第3期601-608,共8页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.31760480
No.31640061)
关键词
3-蒈烯
铜绿假单胞菌
抑菌活性
抑菌机理
细胞膜
3-carene
Pseudomonas aeruginosa
antibacterial activity
antibacterial mechanism
cell membrane
