摘要
目的观察叉头基因-转录因子3a(FOXO3a)与基质金属蛋白酶-9(MMP?9)在子宫肌瘤中的表达情况,探讨二者在子宫肌瘤中的临床意义。方法选取65例子宫肌瘤患者的术后子宫肌瘤组织及正常子宫组织标本,利用和MMP?9基因表达水平,免疫组织化学法检测FOXO3a和MMP?9蛋白表达,分析FOXO3a和MMP?9表达与子宫肌瘤患者临床病理特征的关系。结果与肌瘤旁组织相比,FOXO3a和MMP?9 mRNA在子宫肌瘤组织中表达差异有统计学意义(P<0.05);子宫肌瘤组织中FOXO3a阳性检出率明显低于正常肌瘤旁组织(P<0.05),MMP?9阳性检出率明显高于正常肌瘤旁组织(P<0.05);FOXO3a和MMP?9的表达与患者的年龄、体质指数(BMI)、肌瘤数量及有无乳腺疾病均不具有相关性,差异无统计学意义(P>0.05),与患者经期状况、肌瘤直径明显相关(P<0.05);子宫肌瘤组织中FOXO3a和MMP?9之间呈明显负相关性(r=-0.668,P<0.05)。结论 FOXO3a和MMP?9在子宫肌瘤组织中均异常表达,可能参与调控子宫肌瘤的发生发展等过程,为在临床上靶向治疗子宫肌瘤提供一定的参考方法。
Objective To observe the expression levels of forkhead transcription factor 3 a(FOXO3 a) and matrix metalloproteinase?9(MMP?9)in uterine myoma,and investigate the clinical significances of these two factors in uterine myoma. Methods Uterine myoma tissues and normal uterine tissues from 65 patients with uterine myoma were collected.The expression levels of FOXO3 a and MMP?9 genes in uterine myoma tissues and adjacent tissues were detected by qRT?PCR;the expression levels of FOXO3 a and MMP between FOXO3 a and MMP?9 expression levels and clinicopathological characteristics of patients with uterine myoma were analyzed. Results The expression levels of FOXO3 a and MMP different from those in para myoma tissues(P<0.05);the positive detection rate of FOXO3 a in uterine myoma tissues was significantly lower than that in normal para myoma tissues(P<0.05),and the positive detection rate of MMP?9 was significantly higher than that in normal para myoma tissues(P<0.05). The expression levels of FOXO3 a and MMP?9 were not correlated with age,body mass index(BMI),tumor number and breast disease(P>0.05),but with menstrual status and tumor diameter(P<0.05);there was a significant negative correlation between FOXO3 a and MMP?9 in uterine myoma tissues(r=-0.668,P<0.05). Conclusion FOXO3 a and MMP?9 were abnormally expressed in uterine myoma tissues,which might participate in the occurrence and development of uterine myoma.
作者
张杭
刘富君
单青
张琳瑛
肖芬球
ZHANG Hang;LIU Fu-jun;DAN Qing;ZHANG Lin-yin;XIAO Fen-qiu(Department of Obstetrics and Gynecology,the Fifth Affiliated Hospital of Southern Medical University,Guangzhou,Guangdong 510900,China)
出处
《热带医学杂志》
CAS
2019年第2期170-173,180,262,共6页
Journal of Tropical Medicine
