摘要
本研究探讨一氧化氮 (NO)在慢性病贫血 (ACD)发病中的作用及对铁代谢影响的机制 ,为ACD的防治提供实验依据。在建立传统的类风湿性关节炎大鼠动物模型的基础上 ,通过反复注射弗氏完全佐剂 ,建立ACD大鼠动物模型。利用此模型观察不同处理组NO浓度的改变与贫血及铁代谢的关系。结果表明 :ACD组血清NO浓度和一氧化氮合酶 (NOS)活性显著增高 ,贫血明显 ,血清铁、总铁结合力、转铁蛋白饱和度、红细胞内铁蛋白、骨髓血细胞转铁蛋白受体、骨髓内铁及肝脏顺乌头酸酶活性降低 ,血清铁蛋白及肝脏储铁增加 ,与对照组比较差异显著 ;用NOS抑制剂后 ,NO和NOS水平降低 ,贫血改善 ,铁代谢指标介于ACD与正常对照组之间 ,统计学处理有显著性差异。结论 :NO参与了ACD的发病及ACD时铁代谢的调节 ,为从NO对铁代谢影响角度进一步认识ACD的发病机制提供了实验依据 ;及早降低NO水平 ,有利于阻止贫血的发展 。
To explore the role of nitric oxide (NO) in the pathogenesis and effect on regulation of iron metabolism in anemia of chronic disease (ACD) and provide experimental evidence for prevention and treatment of ACD. On the basis of traditional animal model of rheumatoid arthritis, an ACD rat model was established by repeated injection of Freund′s complete adjuvant. The relationship between NO concentration and iron metabolism was observed in ACD rats with and without NO synthase inhibitor, L NAME, (N ω nitro L arginine methyl ester L NAME). The results showed that anemia was induced in the rat model. In the ACD group, NO concentration and NO synthase activity in serum increased; iron, total iron binding capacity (TIBC) and transferrin saturation (TS) in serum and ferritin in erythrocytes (rFn) decreased; transferrin receptor (TfR) and iron in bone marrow cells decreased; ferritin in serum and iron in liver increased and meanwhile the acotinase activity in liver decreased. After administration of L NAME, anemia was improved, when NO, NO synthase activity, liver iron and serum ferritin decreased, but serum iron, TS, TIBC, rFn, TfR, iron in marrow cells and liver acotinase activity elevated. The levels of parameters for iron metabolism in ACD+L NAME group were situated between ACD and control groups. It is concluded that NO palys an important role in pathogenesis of ACD and influences the regulation of iron in ACD. Decrease of NO level as early as possible will benefit to block the development of anemia, that will provide a new strategy of therapy for ACD.
出处
《中国实验血液学杂志》
CAS
CSCD
2003年第4期385-389,共5页
Journal of Experimental Hematology
基金
纽约中华医学基金会资助
关键词
一氧化氮
慢性病贫血
铁代谢
nitric oxide
anemia of chronic disease
iron metabolism
