摘要
目的：探索铁代谢平衡中铁效应元件结合蛋白（ＩＲＥ－ＢＰ）的调节机制。方法：用低铁饮食建立大鼠营养性缺铁性贫血模型，分析缺铁性贫血发生过程中ＩＲＥ－ＢＰｍＲＮＡ的表达水平及顺乌头酸酶活性的变化。结果：ＩＲＥ－ＢＰｍＲＮＡ表达水平在缺铁性贫血发生、发展过程中无明显变化，而顺乌头酸酶活性明显降低，再经硫酸亚铁处理后活性明显升高。结论：ＩＲＥ－ＢＰ可能通过其内部［Ｆｅ－Ｓ］基团变构的翻译后调节与转录后调节共同参与铁代谢平衡的调控。
Objective:To explore the regulatory mechanism of iron responsive element binding protein(IREBP) in iron metabolism.Methods:The expression level of IREBP mRNA and the aconitase activity were studied in iron deficiency anemia (IDA) rat model.Results:No distinct change of IREBP mRNA expression was observed in iron deficiency rats.The aconitase activity of liver cytoplasmic protein was much lower in iron deficiency rats than that in controls and treatment of liver cytoplasmic protein with FeSO4 resulted in a strong stimulation of the enzymatic activity.Conclusion:Both posttranslational and posttranscriptional regulation of IREBP may take part in iron metabolism balance.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1997年第11期563-565,共3页
Chinese Journal of Hematology
基金
中国博士后科学基金
