摘要
为了探索检测Ph+ /bcr abl+ 急性淋巴细胞白血病 (ALL)患者的微小残留病变 (MRD)的简便而灵敏的方法 ,对 84例初治ALL患者和其中的缓解期患者的骨髓分别用细胞遗传学、流式细胞术和巢式RT PCR检测。结果表明 ,缓解期患者骨髓中不存在Ph′染色体 ,巢式RT PCR方法检测到 11/ 14例缓解期患者存在MRD(bcr abl融合基因 ) (阳性率 78 5 7% ) ,而流式细胞术检测到 5 / 14的缓解期患者阳性 (阳性率 35 71% )。巢式PCR的灵敏度达到 10 -6- 10 -7水平 ,流式细胞检测的灵敏度达到 10 -4- 10 -5水平。结论 :Ph染色体核型分析技术检测MRD不够灵敏 ,而巢式RT PCR检测MRD较流式细胞技术检测灵敏度更高 ,且更易于开展应用。
To explore a simple and sensitive method to detect minimal residual disease (MRD) in Ph +/bcr abl + ALL patients, the bone marrow samples from 84 de novo ALL patients were detected by cytogenetic analysis, nested PCR and flow cytometry (FCM). Cytogenetic analysis method is used to detect Ph chromosome, nested PCR and FCM are used to detect bcr/abl mRNA and an abnormal B cell differentiation pattern in de novo and complete remission (CR) patients, respectively. The results showed that Ph chromosome has not been found in 14 cases of CR; bcr/abl fusion gene was detected in 11 of 14 CR patients by nested PCR (78.57%) and bcr/abl fusion gene was positive in 5 of 14 in CR patients (35.71%) by FCM. The sensitivity of nested PCR was 10 -6 -10 -7 , and that of FCM was 10 -4 -10 -5 . It is concluded that the cytogenetic analysis is not sensitive for MRD detection, and the sensitivity of nested PCR is higher than that of FCM in detecting MRD.
出处
《中国实验血液学杂志》
CAS
CSCD
2003年第4期372-375,共4页
Journal of Experimental Hematology
