摘要
目的应用神经营养因子(NGF)观察大鼠坐骨神经离断再吻合后运动功能和病理变化,验证外周神经损伤后的NGF对神经元存活及再生的促进作用。方法采用大鼠右侧坐骨神经在梨状肌下缘10mm处完全切断,将两断端用10-0无损伤缝线,缝合神经外膜对称缝合4针,实验分为NGF组和生理盐水对照组。损伤后,每日给肌注NGF(5mg/L共20μl)和生理盐水(20μl)。结果诱发电位判断运动电位(MEP)损伤后30d观察,治疗组(5.8±1.7)ms,对照组(17.5±2.4)ms,治疗组比生理盐水对照组潜伏期缩短(P<0.05,t=43.5)。感觉电位(SEP)损伤后30d观察,治疗组(9.4±2.8)ms,对照组(19.1±2.7)ms,治疗组比对照组潜伏期缩短(P<0.05,t=5.1)。光镜观察髓鞘肿胀变性,炎细胞浸润再生纤维增多。电镜:损伤组脱髓鞘变化轴浆基质变性,线粒体肿胀较重,治疗组比对照组病理变化程度减轻,但仍有脱髓鞘和结构排列紊乱等变化。结论NGF可促进神经膜细胞增殖分化,为神经再生提供有效的微环境。
Aim To observe the changes of motor function and pathology of sciatic nerve after transverse injury and verify the auxo action of nerve growth factor(NGF) on the survival and regeneration of neurons after external peripheral nerve injury.Methods The right lateral sciatic nerves of 30 Wistar rats were broken on the 10 mm inferior border of piriformis muscle,suturing epineurium symmetrically with 10 0 atraumatic silk for 4 needles,and then the animals were randomly divided into 2 groups of 15 rats:NCF group injected with 20 μ l NGF of 5 mg/L, and control group injected with 20 μ l saline every day.Results The latency of motor evoked potentials(MEP) was (5.8± 1.7)ms in the NGF group shorter than that in the control group [(17.5± 2.4)ms] at 30 days after injury (t=43.5, P< 0.05).The latency of sensory evoke potential(SEP) in the NGF group was (9.4± 2.8)ms also shorter than that in the control group[(19.1± 2.7)ms] at 30 days after injury( t=5.1, P< 0.05).Under the light microscope,myelin sheath showed swelling degeneration and regenerated fibers were increased in number.Under the electron microscope in the injured group,demyelination showed ax plasma base degeneration and mitochondria swelled seriously.The pathological symptom of the NGF group was lighter than that of the control group,still with demyelination and the disorder of structure arrangement.Conclusion NGF significantly promotes the cell proliferation and differentiation providing an effective microenvironment for the nerve regeneration. <P>
出处
《中国临床康复》
CSCD
2003年第17期2396-2397,T001,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家重点基础研究项目(G1999054206)~~
