摘要
目的 联合染色体G显带和间期荧光原位杂交技术 (interphasefluorescenceinsituhybridization ,I -FISH)对急性早幼粒细胞白血病 (acutepromyelocyticleukemia ,APL)患者进行早期诊断和治疗后微小残留病的监测 ,同时对两种方法的灵敏度进行比较。方法 应用染色体G显带技术和I -FISH对 2 0例APL患者进行遗传学检测 ,包括 13例初诊患者和 8例治疗后获完全缓解的患者 (其中有初诊中的 1例 )。结果 ① 13例初诊病例中 ,9例染色体G显带检测t( 15 ;17)阳性 ,2例阴性 ,2例因分裂相缺乏无法分析结果 ,阳性检出率为 9/13 ( 69 2 %) ;I -FISH检测PML/RARa融合基因 13例为阳性 ,阳性检出率为 13 /13 ( 10 0 %) ,细胞阳性率为 76%～ 10 0 %,平均 91 3 %。② 8例治疗后血液学获完全缓解病例 ,染色体G显带后核型分析 6例正常 ,2例无分裂相可供分析 ;I -FISH检测 7例均为阴性 ,1例阳性。结论 联合运用染色体G显带和I
Objective To study chromosomal G-banding and interphase fluorescence in situ hybridization for early diagnosis and MRD monitoring after treatment,and to compare their sensitivity. Methods 20 cases of APL patients, comprising of 13 cases at initial diagnosis and 8 cases at complete remission (including 1 cases once analyzed at initial diagnosis),were analyzed by chromosomal G-banding and I-FISH. Results ①Among the 13 cases at initial diagnosis, 9 cases were found to have positive t(5;17),2 had no chromosomal G-banding and another 2 failed to analyze for absence of metaphase. In contrast, 13 cases were found with PML/RAR α fusion gene,with positive rate varying from 76% to 100% (mean value:91 3%). Chromosomal G-banding presented the t(15;17) translocation in 9/13(69 2%) while I-FISH showed the PML/RAR a gene rearrangement in 13/13(100%). ②8 cases in complete hematological remission were studied with chromosomal G-banding. Results showed that karyotypes of 6 cases were normal and 2 had no metaphase for analysis. 7 cases showed negative I-FISH results and 1 was positive. Conclusion I-FISH and cytogenetic analysis can be used to improve the diagnosis and MRD monitoring in APL.
出处
《广东医学》
CAS
CSCD
2003年第7期708-710,共3页
Guangdong Medical Journal
基金
广东省自然科学基金资助项目 (编号 :0 2 0 0 86)
