摘要
采用高效液相色谱法为定性、定量手段 ,研究了氯霉素在健康鲈鱼体内的药代动力学及在组织中的残留消除规律。采用MCP KP自动化药动学分析程序对数据进行分析。结果表明 ,鲈鱼单剂量口服氯霉素后 (80mg/kg) ,血药经时过程符合一级吸收一室模型 ,其理论方程为 :C血液 =30 815(e- 0 0 6 14t-e- 0 6 6 2t) ;主要动力学参数如下 :Ka 为 0 6 6 2h ,T1/2Ka为 1 0 4 7h ;Cmax为 2 1 0 93μg/ml、Tmax为 3 96 1h、AUC为 4 38 31mg/L·h ;Kel为 0 0 6 14h、T1/2k为 11 2 93h。组织中氯霉素的经时过程均符合一级吸收二项指数方程。鲈鱼多剂量 (4 0mg/kg)给药后药物在组织中的消除可用以下方程描述 :C血液 =3 4 0e- 0 4 0 3t、C肌肉 =4 342e- 0 4 58t、C肝脏 =2 15 2e- 0 2 39t、C肾脏 =6 4 15 5e- 1 4 43t。按照消除方程 ,氯霉素在组织中达到 0 0 1μg/ml所需要的时间在血液、肌肉、肝脏、肾脏组织中分别为 :14 16 4、13 2 6 1、2 2 4 76、6 0 75d。
The pharmacokinetic and residual characteristics of Chloramphenicol in healthy perch were studied by using the High Performance Liquid Chromatography.Data were analyzed with the pharmacokinetic computer program MCP KP.The results showed that the plasma concentration time course of Chloramphenicol can be described by a one compartment open model with the first order absorption after oral administration(80 mg/kg).Its theoretical equation was as follows: C blood =30.815( e -0.061 4t -e -0.662t ); and the main pharmacokinetic parameters were those: K a was 0.662 h, T 1/2K a was 1.047 h; C max was 21.093 μg/ml, T max was 3.961h,AUC was 438.31 mg/L·h,Kel was 0.061 4h, T 1/2k was 11.293h.The kinetics process of Chloramphnicol in tissues can all be described by the biexponential equation with the first order absorption.The elimination characteristics of multiple dose administration(40 mg/kg)can be described by the equations as follows: C blood =3 40 e -0.403t ?C muscle =4.342e -0.458t ?C liver =2.152 e -0.239t ?C kidney =64.155 e -1.443t .According to the equations ,the time taken by the concentrations of Chloramphnicol dropping to 0.01 μg/ml in blood,muscle,liver,and kidney was 14.164,13.261,22.476,6.075 days respectively.
出处
《海洋水产研究》
CSCD
2003年第2期45-50,共6页
Marine Fisheries Research
基金
农业部项目 (编号渔 95 B 0 0 0 1 0 2 )
中国海洋大学海水养殖教育部重点实验室 (2 0 0 2 1 1 )共同资助
关键词
氯霉素
鲈鱼
药代动力学
残留
Chloramphenicol Perch Pharmacokinetics Drug residue
