摘要
目的:观察死于吸入性窒息与肺透明膜病的围产儿肺组织内皮素 - 1(ET- 1)、C- m yc与 p5 3表达的变化 ,探讨其与肺组织缺氧性损伤的关系 ,从分子水平对缺氧围产儿肺组织的病变机制进行研究。方法 :采用免疫组织化学方法 ,检测了死于吸入性窒息患儿 30例 ,肺透明膜病患儿 2 2例肺组织中 ET- 1、C- m yc、p5 3这 3项指标的表达情况 ,并与其他非呼吸系统疾病死亡病例肺组织进行对照。结果:(1)在吸入性窒息和肺透明膜病肺组织中 ,ET- 1分布在肺泡壁上皮细胞和肺泡壁毛细血管内皮细胞 ,C- m yc、p5 3则主要分布于肺泡壁上皮细胞。ET- 1、C-myc、p5 3阳性率吸入性窒息肺组织中分别为 70 .0 0 %、5 6 .6 7%、6 0 .0 0 % ,肺透明膜病肺组织中分别为 72 .73%、5 9.10 %、6 3.6 4 % ,分别与对照组肺组织相比 ,差异均有统计学意义 (P <0 .0 5 )。(2 )吸入性窒息与肺透明膜病 3项指标的阳性率差异无统计学意义 (P >0 .0 5 )。结论 :ET- 1、C- myc、p5 3与肺组织缺氧性损伤密切相关。
Objective: To investingate the effect of hypoxia on expression of endotheliosin(ET 1)?C myc and p53 on fetal lung to explore the possible molecular biology of hypoxia. Methods: The tissue sections from 30 cases of asphyxia and 22 neonatal pulmonary hyaline membrane diseace and 20 relatively nomal human pulmonary tissue were analyzed by immunohistochemistry (LSAB). Result: The positive frequentcy of ET 1?C myc and p53 was 70.00%(21/30)?56.67%(17/30)?60.00%(18/30)?respectively in cases of asphyxia and 72.73%(16/22)?59.10%(13/22)?63.64%(14/21) respectively in hyaline membrane disease. There were statistic significance between asphyxia and normal lung; hyaline membrane disease and normal lung (P<0.05). However ,no difference of the expression of ET 1?C myc and p53 between asphyxia and hyaline membrane disease(P>0.05). Conclusion: ET 1?C myc and p53 have high level expression in asphyxia and hyaline membram disease.
出处
《新疆医科大学学报》
CAS
2003年第3期243-245,共3页
Journal of Xinjiang Medical University
