摘要
目的:探讨睡眠剥夺(SD)对大鼠胃黏膜血流量及胃黏膜损伤的影响. 方法:采用小站台水环境法建立大鼠SD模型,以正常单独笼养组(CC)和大平台实验对照组(TC)作为对照组,应用激光多普勒微循环血流计检测胃黏膜血流量,观察胃黏膜损伤.用放射免疫法(RIA)测定动脉血中内皮素(ET)和降钙素基因相关肽(CGRV)的水平. 结果:在SD3 d、5 d、7 d大鼠胃黏膜血流量与CC组、TC组和sD1d组比较明显下降,血流量分别为(54.2±2.5)、(53.7±3.0)、(48.3±2.5)mv;大鼠胃黏膜损伤与CC组、TC组和SD 1 d组比较明显加重,溃疡指数分别为(29.8±3.2)、(33.7±3.6)、(34.8±3.5).同时SD3 d、5 d、7 d大鼠血浆中ET和CGRP的水平发生明显改变,血浆中ET含量分别为(123±28)ng/L、(139±36)ng/L、(149±38)ng/L;SD 5 d、7 d,CGRP含量分别为(193±32)ng/L、(221±40)ng/L. 结论:SD可导致胃黏膜血流量下降及胃黏膜损伤并且伴随着血浆ET和CGRP含量的变化.
AIM: To observe the changes of gastric mucosal blood flow and gastric mucosal lesions in rats after sleep deprivation. METHODS: Sleep deprivation was induced in male Sprague-Dawley rats housed on small platform over water. Control mice were housed either in normal cages or on large platform over water. Laser Doppler blood flow meter was used to measure the gastric mucosal blood flow, and index of gastric mucosal lesions was quantified. The arterial plasma levels of endotoxin (ET) and calcitonin gene related peptide (CGRP) were measured by radioimmunoassay. RESULTS: Compared with control mice in normal cage, on big platform, or the mice on the 1st day of sleep deprivation, the gastric mucosal blood flow of mice on the 3rd, 5th and 7th day of sleep deprivation (54.2±2.5, 53.7 ± 3.0,48.3±2.5 mv, respectively) were significantly lower. The gastric mucosal injury became more and more serious: the injury indices were 29.8 ± 3.2,3.7 ± 3.6 and 34.8 ± 3.5 on the 3rd, 5th and 7th day of sleep deprivation, respectively. The serum levels of ET were 123 ± 28,139 ± 36 and 149 ± 38 ng/L in mice on the 3rd, 5th and 7th day of sleep deprivation, and the levels of CGRP were 193±32 and 221 ±40 ng/L in the mice on the 5th and 7th day of sleep deprivation. All were significantly higher than those in control mice. CONCLUSION: Sleep deprivation can cause the decrease of gastric mucosal blood flow and the gastric mucosal injury, which are accompanied by the elevation of serum ET and CGRP.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第4期499-503,共5页
World Chinese Journal of Digestology
