摘要
目的 研究食管癌中 p16、nm2 3、PCNA及 p5 3表达及其临床意义。方法 应用免疫组化 (S -P)方法对 10 4例食管鳞状细胞癌组织中 p16、nm2 3、PCNA及 p5 3进行检测。 结果 (1) p16在无淋巴结转移及Ⅰ -Ⅱ期病例阳性表达率 (73.7%、75 .6 % )明显高于有淋巴结转移及Ⅲ -Ⅳ期病例 (4 0 .4 %、4 5 .6 % ,P <0 .0 1) ,且在Ⅰ级鳞癌阳性表达率 (71.8% )与Ⅲ级鳞癌 (4 1.9% )之间也有统计学差异 (P <0 .0 5 )。 (2 )nm2 3在有淋巴结转移及Ⅲ -Ⅳ期病例阳性表达率 (4 4 .7%、5 2 .5 % )明显低于无淋巴结转移及Ⅰ -Ⅱ期病例 (82 .5 %、82 .2 % ,P <0 .0 1)。(3)PCNA在Ⅰ级鳞癌中表达率 (4 8.7% )明显低于Ⅲ级鳞癌 (80 .6 % ,P <0 .0 1)。 (4 ) p5 3与肿瘤分级、侵袭、转移及临床分期之间无相关性 (P >0 .0 5 )。结论 p16及nm2 3可作为判定患者预后的重要指标。
Objectives To investigate the relationship between expression of p16,nm23,PCNA and p53,and the their clinical significance in esophageal cancerous tissues.Methods Immunohistochemical method was used to detect the expression of p16,nm23,PCNA and p53 in 104 cases of esophageal squamous cell carcinoma.Results (1)The positive rate for p16 was significantly higher in cases without lymph node involvement(LN-)(73.7%) and with carcinoma stage(Ⅰ-Ⅱ)(75.6%) than those with lymph node involvement(LN+)(40.4%) and carcinoma stage(Ⅲ-Ⅳ)(75.6%,P<0.01),and there was statistical difference between cases with differentiation Ⅰ and Ⅲ(P<0.05).(2)The positive rate for nm23 was significantly lower in LN(+) cases(44.7%) and with carcinoma stage(Ⅲ-Ⅳ)(52.5%) than those in LN(-) cases(82.7%) and with carcinoma stage(Ⅰ-Ⅱ)(82.2%)(P<0.01).(3)The positive rate for PCNA was signigicantly lower in cases with differentiation(Ⅰ)(48.7%) than those with differentiation(Ⅲ) (80.6%)(P<0.01).(4)p53 is not correlated with the invasion,metastasis and differentiation of tumor(P>0.05).Conclusions These findings indicate that p16,nm23 are important prognostic markers in esophageal carcinoma. [
出处
《宁夏医学杂志》
CAS
2003年第6期328-330,共3页
Ningxia Medical Journal
关键词
食管肿瘤
基因表达
免疫组化
预后
Esophageal neoplasms
Gene expression
Immunohistochemistry
Prognosis
