摘要
目的 研究尿激酶 (uPA)、尿激酶受体 (uPAR)在肾癌中的表达特征 ,评估其在肾癌恶性生长方式中的作用。方法 选取 4 0例不同分化程度及临床分期的肾癌组织和 10例正常肾组织 ,分别以抗uPA单克隆抗体和抗uPAR多克隆抗体 ,用SUPERVISION法进行免疫组织化学染色 ,结果进行统计学处理。结果 uPA、uPAR的表达程度与肾癌细胞的分化程度及临床分期有关 ,分化越差 ,临床分期越晚 ,则表达程度越高。正常肾组织未见uPA、uPAR的表达。uPA、uPAR染色主要定位于肿瘤细胞和血管内皮细胞 ,uPA还见于癌周组织 ,且表达高于癌组织。在肿瘤细胞增殖密集区域 ,uPA、uPAR阳性的肿瘤细胞比例较低 ,而在肿瘤细胞生长稀疏、肿瘤血管增殖旺盛的区域 ,阳性细胞比例较高。结论 uPA、uPAR的共同表达是浸润性生长的肾癌的特征之一。其表达程度可作为判断肾癌浸润性的重要参考指标。在肾癌组织中 ,uPA表达弱于癌旁组织。uPA、uPAR的表达对肾癌的浸润。
Objective To explore the expressions of urokinase and urokinase receptor and their role in the development of renal cancer.Methods Immunohistochemical staining was made monoclonal and polyclonal antibodies through Supervision method in 40 cases of renal cancer tissues at different degrees and 10 cases of normal renal tissues,and the results were calculated and compared.Results The degrees of the expressions of urokinase,urokinase receptor were related to the differential degrees of renal cancel cells and the different clinical stages.The poorer the differentiation was,the later the clinical stage was,and then the higher the degree of the expression.No similar expressions were found in the normal renal tissues.The staining was mainly located in the tumor cells and the vascular endothelial cells.The expressions of urokinase was also found in the peripheral tissues of cancers and higher in degree than those in the cancer tissues.Compared with the dense area of multiplication of tumor cell,the area of less multiplication of tumor cells but of greater multiplication of tumor vessels had more positive tumor cells.Conclusion The expressions of urokinase and urokinase receptor are the main characteristics of infiltrative renal tumors and may play an important role in the invasion of renal cancer.
出处
《临沂医学专科学校学报》
2003年第3期165-168,共4页
Journal of Linyi Medical College
