摘要
观察肺癌ｎｍ２３／ＮＤＰＫ、整合蛋白、Ⅳ型胶原酶、尿激酶（ｕＰＡ）的表达与肺癌转移的相关性。方法以ＡＢＣ免疫组织化学染色，对不同转移力（由低到高依次为ＰＬＡ－８０１Ｃ、Ａｎｉｐ９７３、ＰＬＡ－８０１Ｄ及ＰＬＡ－８０１ＤＬ）４株人肺癌裸鼠移植瘤及８７例人肺癌临床标本作上述４种分子表达的观察，并在裸鼠体内观察整合蛋白β１抗体及其配体ＲＧＤ多肽对移植瘤生长及转移的影响。结果（１）人肺癌移植瘤与临床标本均呈ｎｍ２３／ＮＤＰＫ高表达，且与淋巴结转移无显著相关。（２）４株移植瘤总体上呈β１亚族整合蛋白低表达，且与淋巴结转移无显著相关。β１抗体及ＲＧＤ多肽对ＰＬＡ－８０１ＤＬ移植瘤的转移无明显影响。（３）人肺癌移植瘤及临床标本中Ⅳ型胶原酶的表达与其转移力呈显著正相关。（４）Ａ－ｎｉｐ９７３及ＰＬＡ－８０１ＤｕＰＡ阳性，而ＰＬＡ－８０１ＤＬ及ＰＬＡ－８０１Ｃ则ｕＰＡ阴性。结论肺癌中Ⅳ型胶原酶的表达与其转移力呈显著正相关；ｎｍ２３呈高表达，整合蛋白呈低表达，ｕＰＡ在不同瘤株的表达不一，但均与其转移无显著相关。
Objectives To observe the relationship between the metastasis of human lung carcinoma and the expressions of nm23/NDPK, integrins, type Ⅳ collagenase and uPA. Methods By ABC immunohistochemical staining, we observed the expressions of nm23/NDPK, integrins, type Ⅳ collagenase and uPA in four strains of subcutaneous transplanted tumors and 87 surgical specimens for human lung carcinoma. The metastatic potential of four xenografts varied from low to high among PLA 801C, Anip973, PLA 801D, PLA 801DL. The effects of β 1 antibody and the RGD peptide on the PLA 801DL xenograft were studied in the nude mice. Results High expression of nm23/NDPK was observed in the four xenografts and specimens from the 87 patients. No association was found between expression of nm23/NDPK and lymph node metastasis. Low expressions of β 1 subfamily integrins were observed in the four xenografts. β 1 subunit antibody and RGD peptide had no effect on metastasis of PLA 801DL xenograft. The expressions of type Ⅳ collagenase were positively correlated with their metastatic capacity in the four xenografts and surgical specimens from the 87 patients. Anip973 and PLA 801D showed positive staining to uPA, while PLA 801DL and PLA 801C were negative. Conclusions The expression of type Ⅳ collagenase is positively correlated with the metastatic capacity of lung carcinoma. Although high expression of nm23/NDPK, low expression of integrins, and various expression of uPA were observed, these were not correlated with metastatic capacity of lung carcinoma.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第8期597-600,共4页
National Medical Journal of China
基金
重庆市中青年科技专家基金
