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NaCl胁迫和外源ABA对枸杞愈伤组织膜脂过氧化及抗氧化酶活性的影响 被引量:34

Effect of salt stress and abscisic acid on membrane-lipid peroxidation and resistant-oxidation enzyme activities of Lycium barbarum callus
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摘要 不同浓度NaCl胁迫下,枸杞愈伤组织生长量下降,质膜相对透性和丙二醛 MDA 含量增大,且二者呈正相关;过氧化酶 POD 和超氧化歧化酶 SOD 活性升高,但对POD同工酶酶带无影响,仅使酶带染色程度加深.加入ABA后,SOD、POD活性升高,说明盐胁迫下ABA可以延缓盐胁迫对膜的伤害,从而提高枸杞的耐盐性. The material salttolerance Lycium barbarum calli was studied under the different NaCl concentration .The results indicated that the calli growth rate decreased treated with ABA and NaCl, while MDA and membrane permeability increased with the increase of NaCl concentration .There were positive correlation between them. NaCl and ABA treatment stimulated SOD and POD activities, but did not alter the pattern of POD isoenzymes .SOD and POD activities increased significantly treated with ABA. The results showed that the alleviation of salt injury in Lycium barbarum calli by treating with ABA could be associated with the enhancement of protective ability of the cell.
出处 《西北植物学报》 CAS CSCD 2003年第5期745-749,共5页 Acta Botanica Boreali-Occidentalia Sinica
基金 国家重点基础研究发展规划项目 G1999011704
关键词 NACL胁迫 外源ABA 枸杞 愈伤组织 膜脂过氢化酶 抗氢化酶 茄科 Lycium barbarum L. calli NaCl stress ABA SOD POD membrane permeability MDA
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