摘要
以桔梗茎段为外植体,通过不定芽诱导与生根培养,获得了再生植株;以桔梗茎、叶为外植体,研究愈伤组织的诱导与分化。结果表明:茎段不定芽诱导培养基以MS+6-BA 1.0 mg/L+NAA 0.05 mg/L最佳,生根培养基以1/2MS+IAA 1.0 mg/L+NAA 0.5 mg/L最佳;茎、叶外植体均可成功诱导愈伤组织,其中以茎诱导率最高,最适培养基MS+2,4-D 1.0 mg/L+6-BA 0.2 mg/L。愈伤组织均可分化培养成完整植株,为桔梗优良株系的繁育和利用提供了有效途径。
With Platycodon grandiflorum stem as explants,the regenerated plants were obtained by adventitious buds inducing and rooting. Callus induction and differentiation were studied using stems and leaves from aseptic seedlings as explants. Results showed that optimal medium for adventitious buds induction of stems was MS + 6-BA 1. 0mg/L + NAA 0. 05mg/L. The testtube plantlet could root in the medium 1/2MS + IAA 1. 0mg/L + NAA 0. 5mg/L. Callus that were induced from two kinds of explants and inductivity of stem explants was the highest in medium MS + 2,4-D 1. 0mg/L + 6-BA 0. 2mg/L. All kinds of explants from callus were differentiated. The rapid proliferation of Platycodon grandiflorum by tissue culture method could be established,which would provided effective ways for the breeding of excellent varieties and utilization of Platycodon grandiflorum.
出处
《农业工程》
2014年第4期158-161,共4页
AGRICULTURAL ENGINEERING
基金
湖南农业大学大学生科技创新项目(项目编号:XCX13121)
关键词
桔梗
再生植株
愈伤组织
Platycodon grandiflorum,Plant Regeneration,Callus
