摘要
以7年生文冠果新抽嫩枝为试材,探讨文冠果萌发芽超低温玻璃化法保存技术.结果表明:文冠果超低温玻璃化法保存程序:采用继代培养45d的萌发芽,切取2~3mm,于0.5mol/L蔗糖浓度的MS培养基内预培养2d,60%PVS2室温处理5min,100%PVS2 0℃下处理30min,迅速投入液氮,48h后,37~40℃水浴快速化冻2min,1.2mol/L蔗糖的MS培养液洗涤20min.将洗涤过的萌发芽转移至MS+0.5mg/L BA+0.1mg/L NAA+0.3mg·L-1GA3+30g/L蔗糖+7g/L琼脂的恢复培养基上恢复培养.
Cryopreservation of the shoot of Xanthoceras Sorbifolia Bunge by vitrification was studied. e procedure and corresponding conditions of conservation were as follows: the shoot were subcul-tured by 45 days and detached the size of 2~3mm shoot precultured on MS medium with 0.5M su-crose for 2 days ,then it was dehyrated with 60%PVS2 Solution for 5 minutes at room temperature, then with 100%PVS2 Solution for 30 minutes at 0℃. The materials were plunged into liquid nitro-gen. After 48h,The material was taken off from liquid nitrogen and thawed in 37- 40℃ 2 minute, then it was cultivated on MS+6-BA 0.5mg·L-1+NAA 0.1mg·L-1+GA 0.3 mg·L-1+sucrose 30g·L-1+agar 7g·L-1.
出处
《内蒙古民族大学学报（自然科学版）》
2015年第5期407-411,共5页
Journal of Inner Mongolia Minzu University：Natural Sciences
关键词
文冠果
玻璃化法
超低温保存
Xanthoceras Sorbifolia Bunge
Vitrification method
Cryop reservation
