摘要
目的:NMDA受体的NR1和NR2B亚基对于痛觉的产生和维持起着关键作用,本文旨在探究NMDA受体在大鼠睾丸痛模型中的表达及可能机制。方法:参照Yoshioka等的方法建立大鼠睾丸痛模型,分实验组和对照组,其中对照组大鼠睾丸注射0.2 ml生理盐水,实验组大鼠睾丸注射0.2 ml 2%醋酸溶液,后进行行为学检测。建模成功后4 h采用Western印迹、RT-qPCR、免疫荧光检测NMDA受体在背根神经节和脊髓背角中的表达。结果:建模后,实验组大鼠逃避反应潜伏期明显降低,于4 h达到最低值[(4.15±0.84) s],显著低于对照组[(12.32±1.05) s,P<0.05]。建模后4 h,RT-qPCR, Western印迹、免疫荧光结果显示:与对照组相比,实验组大鼠背根神经节中NR2B mRNA和蛋白表达明显升高(P<0.05),脊髓背角中NR2B的表达无明显变化;实验组和对照组大鼠的背根神经节和脊髓背角的NR1表达也无明显差异。结论:NMDA受体在大鼠睾丸痛发病过程中具有重要作用,疼痛前期便可通过上调NMDA受体NR2B亚基表达介导外周痛敏形成,介导睾丸疼痛的发生。
Objective:To explore the expression of the N-methyl-D-aspartate(NMDA)receptor in the rat model of orchialgia and its possible mechanisms.Methods:According to Yoshioka’s method,the male rats in the control group were injected with 0.2 ml saline,and those in the experimental group with 0.2 ml 2%acetic acid solution.Then we tested the behavioral responses of the rats and determined the expressions of the subunits NR1 and NR2 B of the NMDA receptor in the dorsal root ganglion and spinal dorsal horn by Western blot,RT-qP CR and immunofluorescence staining.Results:The withdrawal latency was decreased in the model rats,reaching the lowest value at 4 hours after modeling,significantly lower than in the controls([4.15±0.84]vs[12.32±1.05],P<0.05).Compared with the controls,the model rats showed remarkably increased mRNA and protein expressions of NR2 B in the dorsal root ganglion(P<0.05)but not in the spinal dorsal horn at 4 hours.However,no statistically significant difference was found in the expression of NR1 either in the dorsal root ganglion or in the spinal dorsal horn between the two groups(P>0.05).Conclusion:The NMDA receptor plays an important role in pathogenesis of orchialgia in rats.In the early stage of pain,upregulating the expression of the subunit NR2 B of the NMDA receptor can mediate peripheral hyperalgesia and consequently orchialgia.
作者
田昆
余敬威
庄锦涛
周明宽
冯鑫
龙世颖
汪富林
周文良
邓春华
涂响安
TIAN Kun;YU Jing-wei;ZHUANG Jin-tao;ZHOU Ming-kuan;FENG Xin;LONG Shi-ying;WANG Fu-lin;ZHOU Wen-liang;DENG Chun-hua;TU Xiang-an(Department of Urology and Andrology,The First Hospital of Sun Yat-sen University,Guangzhou,Guangdong 510080,China;School of Life Sciences,Sun Yat-sen University,Guangzhou,Guangdong 510275,China)
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2019年第4期296-301,共6页
National Journal of Andrology
基金
国家自然科学基金(81471449和81671449)
广东省科技计划项目(2016A020218007和2016B030230001)
广州市科学研究计划重点项目(201604020189)~~
