摘要
用^(32)P标记钩端螺旋体(钩体)基因组DNA,以Dot-blot和Southern-blot杂交法分析5株钩体DNA同源性。结果表明:问号钩体黄疸出血群赖型017株和601株及七日热群七日热型245株与双曲钩体patoc型Patoc I株、细螺旋体illini型3055株同源程度极低;3株问号钩体有不同程度同源;illini型3055株与各株同源性均低。
Nick translation labellingtechnique was applied to the preparationof two ^(32)P labelled genomic DNA probesfrom L. interrogans serovar Lai strain 017and Leptonema illini strain 3055, respec-tively. Dot-blotting and Southern-blottingwith DNA of five strains leptospira fromdifferent genus and species were performed.The results showed that certain differencesamong the L. interrogans, L. biflexa andLeptonema illini could be detected by en-donuclease assay. L. interrogans strain 017with L. biflexa strain Patoc I and Lepto-nema illini 3055 strain exhibited very littlehomology. L. interrogans strains 017. 601,and 245 from different serogroup and ser-ovar had a high degree of homology. Le-ptonema illini strain 3055 showed little ho-mology with L. interrogans and L.biflexa.Therefore DNA hybridization may bo usedas a tool for the identification and classifi-cation of leptospira.
出处
《华西医科大学学报》
CAS
CSCD
1992年第2期122-125,共4页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
卫生部科研基金
关键词
钩端螺旋体
基因组
DNA
分子杂交
Leptospira
Genomic DNA
Homology
DNA-DNA hybridization
^(32)P-probe
