摘要
目的 探讨运用同种异体软骨细胞复合 Pluronic修复关节软骨缺损的可行性 ,并应用 3H- Td R放射自显影方法鉴别软骨缺损修复的细胞来源。 方法 取同种异体软骨细胞体外培养至第 2代 ,用 3H- Td R标记后复合Pluronic植入兔关节软骨缺损区作为实验组 ,并采用单纯Pluronic植入作为材料对照组 ,不作任何处理组为空白对照组 ,分别于 4、8及 16周取材 ,观察其修复效果 ,并应用放射自显影方法鉴别修复组织的细胞来源。 结果 实验组术后 8周 ,缺损表面可见新生软骨形成 ,术后 16周缺损完全修复 ,表面光滑 ,与周围界限模糊 ,放射自显影证实所修复组织的细胞来源于植入细胞。材料对照组及空白对照组缺损均未见明显修复。 结论 1同种异体软骨细胞复合 Pluronic修复关节软骨缺损是可行的 ;2 3H- Td R标记细胞可作为鉴别细胞来源的一种简便可行的方法。
Objective To investigate the possibility of repairing the cartilage cartilage defect with homogeneous chondrocytes combined with Pluronic. Methods Homogeneous cartiage chondrocytes of adult New Zealand rabbits were harvested and cultured in vitro, which were marked by 3H-TdR and mixed with Pluronic. The medial or lateral condyle defects were made (Φ4mm,extending down to the calcified zone) in 20 rabbits. In the experimental group, the right defects were repaired by homogeneous chondrocytes combined with Pluronic; in the control group, the left defects were repaired by Pluronic only or were left un-repaired. The animals were sacrificed in the 4th, 8th and 16th weeks after operation respectively. The repair results were observed and the cell source of repair tissue was distinguished. Results In the experimental group, the cartilage defects were repaired by the cartilage-like tissue after 8 weeks of operation; the defects were completely filled with mature cartilage tissue, which integrated smoothly with articular cartilage 16 weeks later. In the control group, only a small amount fibrous tissues were seen on the surface of defects. Autoradiographic assessment showed that the repair cells came from the implants, but not from self-chondrocytes. Conclusion It is a good way to repair articular cartilage defects with homograft of tissue engineering cartilage. It is a convenient method to mark with 3H-TdR to discriminate the resource of the repair cells.
出处
《中国修复重建外科杂志》
CAS
CSCD
2003年第3期255-258,共4页
Chinese Journal of Reparative and Reconstructive Surgery
