摘要
采用本地小鹅瘟强毒株C-85 F^2成功地研制了鹅胚化小鹅瘟(简称SEP)和鸭胚化小鹅瘟(简称SGD)疫苗,并对两苗进行了安全试验、无菌试验、效力测定试验和田间免疫试验。结果表明,用SEP或SGD原液2lm肌注成年母鹅均安全;经1次免疫(1:1OO SGD或1:100 SEP每只1ml)或2次免疫(1:100 SGD和1:50 SEP每只各1ml)的成年母鹅种蛋所孵的鹅胚或雏鹅,都具有抵抗小鹅瘟强毒的能力,其保护率达90%;在岳阳、怀化等地田间免疫母鹅的跟踪调查,其抵抗自然感染的保护率为95%。
Goose embryo-treated gosling plague vaccine (SEP) and duckembryo-treated gosling plague vaccine (EGD) were sccessfully made by strongvirus of local gosling plague (C-85-F2). Experiments were made for the vac-cines on safeness, bacteria testing and efficiency. Field immune experiments on alarge scale were also carried out. The results indicated that muscular injectionwith 2 ml SEP or SGD to each goose would cause no problem; goose embryoes orgoslings hatched from the immunized goose eggs with 1 ml 1:100 SEP/1:100 SGDor twice with 1 ml 1:100 SGD and 1:50 SEP had the ability to resist strong virusof gosling plague. 12 d aged goose embryoes and 2~3 d aged goslings could resiststrong virus (C-85-F_2), their protection rate was 87.5%~90.9% respectively.In one field experiment, from 237 000 offsprig of 11 160 immunized geese, undernatural infection conditions, the protection rate was 93%, in another experimentof 8 800 descendants of 350 immunized geese, the protection rate was 95%.
关键词
小鹅瘟
鹅
防治
疫苗
湖南
gosling
avian disease
vaccine
Hunan/gosling plague
