摘要
目的 研究骨形态发生蛋白微球的生物相容性。方法 将壳聚糖与海藻酸钠制成复合微球 ,包裹骨形态发生蛋白 ,采用体外细胞培养技术 ,将微球与细胞一起培养 ,通过对细胞增殖率及ALP活性、细胞微量蛋白的检测 ,观察骨形态发生蛋白微球对培养细胞的影响。结果 骨形态发生蛋白微球对骨髓基质细胞的增殖无明显影响 ,但对其分化及分泌功能均有明显促进作用。结论 骨形态发生蛋白微球生物相容性良好 ,能够提高骨髓基质细胞的体外成骨能力。
Objective To evaluate the biocompatibility between bone morphogenetic protein(BMP) composition and bone marrow stem cell (BMSc).Methods Microspheres were prepared with chitosan and sodium alginate, and BMP was enwrapped in the microspheres. The biocompatibility of the composition was examined using cell-culturing method. The BMSc was cultured in combination with microspheres. The extending speed of the cells, the proliferation and alkaline phosphatase activity were tested.Results There were no inhibition on cellular proliferation of BMSc when it was cultured in combination with microspheres in vitro, but ALP activity increased significantly.Conclusion BMP microspheres possessed satisfactory biocompatibility, and could increase the osteogenic capability of BMSc in vitro.
出处
《中国医师杂志》
CAS
2003年第5期582-584,共3页
Journal of Chinese Physician
基金
国家自然科学基金 (30 2 71 32 0 )
广东省团队项目 (1 0 71 71 - 4 1A1 2J1 5)资助项目
关键词
骨形态发生蛋白
骨髓基质细胞
生物相容性
Bone morphogenetic protein
Bone marrow stem cell
Biocompatibility
