摘要
用贴壁法体外分离骨髓基质干细胞 ,实验组 5 氮胞苷诱导 ,对照组加入等量培养基 ,用相差显微镜和免疫组织化学方法观察、鉴定培养细胞。探讨成年狗骨髓基质干细胞体外诱导分化为肌细胞的可行性 ,为心肌梗死和心力衰竭的细胞移植治疗提供新的细胞系。结果表明 ,实验组诱导后 4周 ,多数培养细胞由梭形变为杆状 ,免疫组织化学检查结蛋白、平滑肌肌动蛋白和肌钙蛋白I染色阳性 ;对照组多数培养细胞为梭形 ,免疫组织化学检查结蛋白染色弱阳性 ,平滑肌肌动蛋白染色弱阳性 ,肌钙蛋白I染色阴性。结果提示 ,成年狗骨髓基质干细胞体外可诱导分化为肌源性细胞。
To observe the possibility of differentiation of bone marrow stromal stem cells of adult dog into myogenic cells. In a hope of providing a new cell line for treatment of myocardial infarction and heart failure. Bone marrow stromal stem cells were isolated by their adherence to plastic in vitro . 5 azacytidine was used to induce stem cells to differentiate into cardiomyotes in the experimental group, and same volume of culture media was used in the control group. The cultured cells were exarnined with inverted contrast phase microscope and immunochemistry. 4 weeks after induction, the shape of most of cultured cells changed from fusiform into rod like contiguration in experimental group, immunohistochemistry showed that the induced cells were positive for Desmin, α smooth muscle actin, and Troponin I. The shape of cultured cells remained fusiform in control group, immunohistochemistry showed that they were mildly positive for Desmin and α smooth muscle actin and negative for Troponin I. Adult canine bone marrow stromal stem cells were able to differentiate into myogenic cells after induction in vitro .
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2003年第5期436-437,T002,共3页
Medical Journal of Chinese People's Liberation Army
