摘要
目的 研究丙烯酰胺 (AA)对人角质形成细胞的细胞毒性和对DNA的损伤及其可能机制。方法 (1 )人角质形成细胞株HaCaT细胞经AA浓度梯度染毒 44h后 ,以四氮唑盐 (MTT)法检测细胞存活率。 (2 )细胞经AA浓度梯度染毒 ,以彗星试验检测细胞DNA损伤。 (3)细胞经 2 .0 0mmol/LAA和 0 .50mmol/L的细胞色素P 450 (CYP 450 )抑制剂 1 ABT共处理 4h后 ,以彗星试验探索DNA损伤与CYP 450的关系。结果 (1 )细胞毒性试验显示 ,AA染毒 44h后细胞存活率显著下降。 (2 )AA染毒 4h未观察到细胞毒性 ,但各组细胞的DNA较对照组均检测到显著损伤 ,且损伤程度随染毒剂量增大而加重 ,彗尾长度在处理剂量范围内有明显的剂量效应关系 ;细胞经 1 ABT和 2 .0 0mmol/LAA共同处理后 ,拖尾率和尾长为 1 5 .4 %和 (8.2± 2 .0 ) μm ,较 2 .0 0mmol/LAA组 [80 .6 %和 (44.3± 4 .0 ) μm]明显降低 ,差异有显著性 (P <0 .0 1 )。结论 AA对HaCaT细胞具有细胞毒性和基因毒性 ,AA诱导的DNA损伤可能与其经CYP
Objective To investigate the toxic and DNA damaging effect of acrylamide (AA)on human keratinocytes and its mechanism. Methods (1)After the keratinocyte cell line HaCaT cells were exposed to AA with different concentrations for 44 hours,cell survival rate was detected by MTT method.(2)The effects of DNA damage of exposed cells were detected by comet assay.(3)After treating the cells with 2.00 mmol/L of AA plus 0.50 mmol/L of 1 aminobenzotriazole(1 ABT),an inhibitor of cytochrome P 450 enzymes(CYP 450),for 4 hours,the relationship between DNA damage and CYP 450 was studied. Results (1)Cytotoxicity measurement of AA showed that cell survival rate dereased significantly after 44 hour treatment.(2)Cytotoxicity was not detected after 4 hour AA treatment,but significant DNA damage was observed in all treatment groups,and the degree of damage increased with the concentration of AA.Moreover,the tail lengths of comet cells were in dose effect relationship.As for cells treated by 1 ABT with 2 mmol/L AA,comet rate and tail length were 15.4% and (8.2±2.0)μm respectively,which were decreased significantly( P <0.01) when compared with 2 mmol/L AA treatment group[80.6% and (44.3±4.0)μm]. Conclusions Acrylamide has significant cytotoxicity and genotoxicity on HaCaT cells.AA induced DNA damage may be related to the oxidative metabolite(s) of AA through CYP 450.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2003年第2期96-98,F003,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
