摘要
目的了解明胶作为粘膜疫苗佐剂的可能性,动态观察不同浓度明胶对抗SOD-IgA抗体应答的影响。方法以鼠伤寒沙门氏菌SOD作为抗原,分别加不同浓度明胶经灌胃途径免疫小鼠,用间接ELISA法测定免疫后10、15、20、30天血清中及30天后肠液中抗SOD-IgA抗体效价。结果2％明胶组血清及肠液中抗SOD-IgA抗体效价均显著高于无明胶组(P<0.01)。结论明胶可促进IgA抗体应答,且此种促进作用可能与明胶浓度有关。
Objective To explore the possibility that the gelatin as a mucosal adjuvant and to observe the effective of gelatin on IgA generation with mouse. Methods These mouses were divided in 5 groups and immunized by gastric rout with Salmonella typhimurium SOD, which mixed with defferent gelatin concentration respectively. After 10,15,20 and 30 days, the tilers of anti-SOD antibody in serum or in gut juice were assayed respectively by indirect ELISA. Results The results suggested that the tilers distinct between 2% gelatin group and non-gelatin group were remarkable (P < 0.01). Conclusion The gelatin could promote the generation of IgA in mouse which vaccinated by gastric rout. And maybe the promotion was associated with the concentration of gelatin.
出处
《贵州医药》
CAS
2003年第4期304-305,共2页
Guizhou Medical Journal
