摘要
目的 :观察小鼠鼻腔接种伤寒杆菌的 Fe- SOD后血液和粘膜系统的抗体应答。方法 :用 IL- 1作为佐剂 ,将伤寒杆菌的 Fe- SOD经鼻腔接种小鼠 ,检测小鼠血液及肠液中的抗体应答。结果 :当用 IL - 1作为佐剂时 ,经鼻腔接种伤寒杆菌Fe- SOD的小鼠血液和肠液中可产生高水平特异性 Ig G和 Ig A类抗体 ;而腹腔注射仅在血液中产生高水平特异性 Ig G抗体。结论 :用 IL - 1作为佐剂 ,伤寒杆菌 Fe- SOD经鼻腔接种后可引起小鼠粘膜系统和血液中的抗体应答。
Objective:To observe the antibody response induced by intranasal administration of S. typhi Fe-SOD in mice. Methods: The mice were immunized by intranasal administration or abdominal injection of S. typhi Fe-SOD and interleukin-1 (IL-1). The antibody against Fe-SOD in serum and intestinal juice was detected by ELISA. Results: Intranasal immunization induced high levels of IgG and IgA against Fe-SOD in mouse serum and intestinal juice, whereas abdominal injection only induced high levels of IgG in serum. Conclusion: Intranasal immunization with S. typhi Fe-SOD and IL-1 is able to induce antibody response in serum and intestinal mucosa.
出处
《广东医学院学报》
2005年第1期14-16,共3页
Journal of Guangdong Medical College
基金
湛江市科技攻关项目 (ZK0 1 2 3)
