摘要
目的 研究山羊下颌骨牵张成骨术后牵张区成骨细胞的增殖节律性。方法 选取 4 8只健康山羊 ,分为牵张成骨组和对照组 2组 ,牵张成骨组进行牵张成骨术 ,术后 4周 ,分别在 0 :0 0、4 :0 0、8:0 0、12 :0 0、16 :0 0、2 0 :0 0时点处死两组动物 ,并取牵张成骨组右下颌骨颏孔区新骨组织、对照组右侧颏孔区骨组织各 2cm× 2cm×0 .5cm进行原代细胞培养成骨细胞 ,采用流式细胞术对成骨细胞的增殖指数 (PI)进行定量测定 ,并采用余弦分析法对两组细胞增殖的节律性进行分析。结果 牵张成骨组和对照组的PI均可拟合余弦曲线 ,牵张成骨组的调整值和振幅显著高于对照组 (P <0 0 5 ) ,两组峰值相位无显著性差异 (P >0 0 5 ) ,其峰值出现在 19:4 7。结论 牵张成骨可增加成骨细胞的增殖活性 ,牵张后成骨细胞仍具有昼夜节律性变化。
Objective\ The study was to investigate the circadian rhythm of the proliferation index (PI) of the mandibular osteoblast after distraction osteogenesis (DO) by a comparative study to explain the different rhythm characteristics.Methods\ Fourty_eight healthy goats were employed and divided randomly into DO group and control. Four weeks after the right side mandible of DO group was distracted, animals of the both groups were killed at 0:00, 4:00, 8:00, 12:00, 16:00, 20:00 time point subsequently. Samples of 2 cm×2 cm×0.5 cm bone tissue were excised from distracted zone of DO group and the right mental foramen zone of the control. Primary cell culture method was employed to isolate the osteoblast from bone tissue and a flow cytometric analysis was used to detect the PI of osteoblast. The data was analyzed with cosinor rhythmometry method and the different rhythm between the two groups were investigated.Results\ The PI in the two groups demonstrated cosinor waves. The mesor and amplitude in DO group were significant higher compared with the control(P<0 05), but there was no significant difference for the acrophase between the two groups (P>0 05).Conclusion\ The PI of osteoblast after DO showed significant circadian rhythm. It is suggested that DO is helpful to promote osteoblast proliferation and bone regeneration.\;
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2003年第2期144-146,152,共4页
West China Journal of Stomatology
基金
国家自然科学基金资助项目 (编号 30 0 70 81 9)
