摘要
本文首次研究了酸度、温度对对称草酰胺:N-N’-二-邻羧基苯基草酰胺(OBBE)荧光特性的影响。测定了该试剂的荧光量子产率。发现在pH=8.0～9.5的H_3BO_3—NaOH缓冲介质中,硝酸根离子能使OBBE荧光强度定量猝灭。据此建立了同步荧光猝灭法测定硝酸根离子的新体系。OBBE的激发波长为210nm,发射波长为395nm。荧光猝灭值与NO_3^-在0.0028～0.16mg·L^(-1)呈线性关系,方法的检出限为0.0028mg·L^(-1)。实验了多种干扰离子的影响,用于水中硝酸根离子的测定,结果满意。
The effects of acidity and temperature on the fluorescent characteristics of N-N-bis- (O-Carboxylphenyl) -Oxamide (OBBE) have been studied in details. And the quantum efficiency of fluorescence has been measured. In a pH 8-9.5 buffer medium of H3BO3-NaOH, OBBE has a maximum excitation wavelength at 210 nm and a maximum emission wavelength at 395 nm. The nitrate reacts with OBBE and quenches the fluorescent intensity of OBBE. Thus a new sensitive method using the,quenching of synchronous fluorimetry has been developed for the-direct determination of nitrate in water. The linear calibration curve was obtained in the range of 0.002 8 and 0.16 mg.L-1, the detection limit was 0.002 8 mg.L-1. The proposed Method has been applied to the determination of nitrate in water with satisfactory results.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2003年第2期315-317,共3页
Spectroscopy and Spectral Analysis
