摘要
目的 :观察花旗泽仁主要活性成分在大鼠肠道菌群中的代谢情况,研究花旗泽仁主要活性成分在SD大鼠体外肠道菌群的代谢规律并探索其原型成分与代谢产物的相关性。方法 :通过超高效液相色谱-飞行时间质谱仪(UPLC-Q-TOF-MS)分析手段,检测花旗泽仁主要活性成分含量,鉴定代谢产物结构。结果 :①花旗泽仁的主要活性成分人参皂苷Rb1(G-Rb1)、泽泻醇A-24-醋酸酯(Alisol A 24-acetate)和9-羟基-(10E,12E)-十八碳二烯酸(9-HODE)可以被肠道菌群代谢,且肠道菌群对G-Rb1的代谢较快,而Alisol A 24-acetate和9-HODE的代谢速度相比较慢。②本研究确定了G-Rb1的4个代谢产物人参皂苷Rd(G-Rd)、人参皂苷F2(G-F2)、人参皂苷C-K(C-K)和20(S)原人参二醇(Ppd),Alisol A 24-acetate的1个代谢产物泽泻醇A(Alisol A)。结论 :本研究通过体外肠菌代谢实验,明确了花旗泽仁中主要活性成分及其代谢产物在肠道菌群中的代谢规律,并确定代谢产物结构。
Objective:To observe the metabolism of the main active ingredients of Huaqizeren in the intestinal flora of rats,study the metabolism of the main active ingredients of Huaqizeren in SD rats in vitro and explore the relationship between the prototype components and metabolites.Methods:UPLC-QTOF-MS analysis was used to detect the contents of main active components of Huaqizeren and identify the structure of metabolites.Results:①The main active ingredients of Huaqizeren Ginsenoside-Rb1(G-Rb1),Alisol A 24-acetate and 9-hydroxy-octadecadienoic acid(9-HODE)could be metabolized by the intestinal flora,and the metabolism of G-Rb1 in the intestinal flora is faster,while Alisol A 24-acetate and 9-HODE metabolic rate is relatively slow.②The study identified 4 metabolites of G-Rb1,such as Ginsenoside-Rd(G-Rd),Ginsenoside-F2(G-F2),Compound K(C-K)and 20(S)protopanaxadiol(Ppd),only one metabolite of Alisol A 24-acetate was Alisol A.Conclusion:In this study,the metabolism of the main active components and their metabolites in the Huaqizeren was determined by in vitro intestinal metabolism experiments,and the structure of the metabolites was determined.
作者
李佳欣
赵聪
李璐
陈思琦
邢楠楠
张宇驰
吕忠民
葛鹏玲
LI Jiaxin;ZHAO Cong;LI Lu;CHEN Siqi;XING Nannan;ZHANG Yuchi;LYU Zhongmin;GE Pengling(Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;Hongqi Hospital Affiliated of Mudanjiang Medical College,Mudanjiang 157011,Heilongjiang,China;Mudanjiang Medical College,Mudanjiang 157000,Heilongjiang,China)
出处
《辽宁中医药大学学报》
CAS
2019年第6期58-65,共8页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家科技重大专项项目(2012ZX09103201-018)
国家自然科学基金资助项目(81273650)
黑龙江省博士后落地科研启动金项目(LBH-Q15136)
黑龙江省杰出青年科学基金项目(JC2018025)
黑龙江中医药大学研究生创新科研基金项目(2018yjscx002)
