摘要
目的 :从氧化应激和凋亡途径研究罗汉果皂苷对阿霉素心肌损伤大鼠的治疗作用,以为罗汉果皂苷治疗阿霉素心肌损伤提供基础实验理论。方法 :60只清洁级雄性SD大鼠采用一次性尾静脉注射阿霉素18 mg/kg构建阿霉素心肌损伤模型,模型构建成功后按照随机数字法将其分为正常组,模型组以及罗汉果皂苷低、中和高剂量治疗组,每组12只,罗汉果皂苷治疗4周后,收集血清和心脏,检测心肌病理学、心功能、血清SOD和MDA,同时采用Western Blotting和实时荧光定量检测心肌组织中Bax、Bcl-2蛋白和mRNA的表达特点。结果 :HE染色可见正常组心肌纤维排列整齐,模型组则有明显的心肌排列紊乱,与模型组相比,罗汉果皂苷低、中和高剂量有不同程度的减轻;与正常组相比,模型组AST、LDH、CK-MB和MDA明显升高(P<0.05),罗汉果皂苷3个剂量组均可以明显降低上述几个指标(P<0.05);与正常组相比,模型组SOD明显降低(P<0.05),罗汉果皂苷3个剂量组相较于模型组则明显升高(P<0.05);与正常组相比,模型组Bax蛋白和mRNA明显升高(P<0.05),Bcl-2蛋白和mRNA明显降低(P<0.05),与模型组相比,罗汉果皂苷3个剂量组均可以降低Bax(P<0.05),同时升高Bcl-2表达(P<0.05)。结论 :罗汉果皂苷可增强阿霉素心肌损伤大鼠抗氧化能力,抑制心肌细胞凋亡,进而改善心功能。
Objective:To study the therapeutic effect of mogmside on oxidative and apoptotic in adriamycin myocardium injury rat,in order to provide a basic theory for mogmside treating myocardial injury.Methods:60 clean grade male SD rats were established myocardial injury model by treated with a single intravenous injection of adriamycin(18 mg/kg),after the model successful construction,they were divided into the normal group,model group,and mogmside low,medium and high dose treatment group according to the random number method,each group was 12 cases.After 4 weeks of treatment,serum and heart tissue were collected to detect myocardial pathology,heart function,serum SOD and MDA.Meanwhile,Western Blotting and real-time fluorescence quantitative detected the mRNA and proteins expression of Bax and Bcl-2 in myocardial tissue.Results:HE staining showed that the myocardial fibers in the normal group were well-arranged,while in the model group had significant myocardial disorder,compared with the model group,the low,medium,and high doses of mogmside had varying degrees improvement;compared with the normal group,the AST,LDH,CK-MB and MDA in the model group were significantly increased(P<0.05),and the content of the above-mentioned several indexes was significantly reduced in the three mogmside groups(P<0.05);Compared with the normal group,the SOD in the model was significantly lower(P<0.05),with the model group,SOD in the mogmside groups was significantly higher(P<0.05).Compared with the normal group,the Bax protein and mRNA expression in the model group were significantly higher(P<0.05).Bcl-2 protein and mRNA expression were significantly lower(P<0.05),compared with the model group,mogmside could reduce Bax expression(P<0.05),while increase Bcl-2 expression(P<0.05).Conclusion:Mogmside could enhance the anti-oxidation ability and inhibit cardiomyocyte apoptosis in rats with adriamycin-induced myocardial injury,further improve cardiac function.
作者
黄文蔚
洪李锋
郭璠
龚平
伍琼
HUANG Wenwei;HONG Lifeng;GUO Fan;GONG Ping;WU Qiong(The Fifth Hospital of Wuhan,Wuhan 430050,Hubei,China;The Institute of Cardiovascular Diseases of Jianghan University,Wuhan 430050,Hubei,China)
出处
《辽宁中医药大学学报》
CAS
2019年第6期39-42,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
武汉市科学技术局青年科技晨光计划项目(2015070404010215)
