摘要
目的:研究罗汉果甜苷(mogroside,Mog)的保肝作用。方法:将72只小鼠随机分成6组:正常对照组、模型组、联苯双酯(BPD)组(0.6 g.kg-1)、Mog高、中、低剂量组(1.5,0.75,0.38 g.kg-1)。除正常组和模型组外,其余小鼠每日ig给药1次,共7 d,末次给药1 h后各给药组及模型组腹腔注射(ip)0.08%CCl41次(10 mL.kg-1)。造模后12 h,采血测定血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)含量,并观察肝组织病理变化;另取72只Wistar大鼠,随机分成6组:正常对照组、模型组、联苯双酯(BPD)组(0.1 g.kg-1)、Mog高、中、低剂量组(0.8,0.4,0.2 g.kg-1)。除正常对照组外,其他大鼠按2 mL.kg-1(首次5 mL.kg-1)皮下注射(sc)25%CCl4每周2次,连续8周,造成慢性肝损伤,观察罗汉果甜苷对大鼠血清ALT,AST活性,透明质酸(HA)、Ⅲ型前胶原氨基端肽(PⅢNP)、羟脯氨酸(Hyp)含量,肝组织超氧化物歧化酶(SOD)、谷胱甘肽(GSH-Px)活性及丙二醛(MDA)含量的影响,观察罗汉果甜苷对肝组织转化生长因子β1表达的影响及对肝组织病理变化的影响。结果:罗汉果甜苷对CCl4诱导小鼠急性肝损伤,可降低血清中ALT,AST活性,明显减轻肝组织病理变化程度;对CCl4所致大鼠慢性肝损伤,罗汉果甜苷可降低血清中ALT,AST活性;降低HA,PⅢNP,HyP含量;升高肝组织SOD,GSH-Px活性;降低肝组织MDA含量;抑制肝组织转化生长因子β1(TGF-β1)的表达,并明显减轻肝组织病理变化程度。结论:罗汉果甜苷对CCl4所致小鼠急性肝损伤有保护作用;对CCl4所致大鼠慢性肝损伤有防治作用,并有一定的抗肝纤维化作用。
Objective： To study the hepatoprotective effect of mogrosides （Mog）. Method： Seventy-two mice were randomly divided into six groups： the normal group, the model group, bifendate （BPD） group （0.6 g·kg-1）, high dose Mog group （1.5g·kg-1）, middle dose Mog group （0.75 g·kg-1） and low dose Moggroup （0.38 g·kg-1 ）. Except for normal group and model group, the other mice were orally administered corresponding drugs once per day for seven days. After the last administation, the treated groups and model group were ip given 0.08% CC14 （10 mL ~ kg-1 ） once to produce acute liver injury. The serum was collected after 12 h to detect level of aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, and the histological changes of liver were observed. Seventy-two Wistar rats were randomly divided into six groups： the normal group, the model group, BPD group （0. 1 g·kg-1 ） , high-dose Mog group （0.8g·kg-1 ） , middle-dose Mog group （0.4g·kg-1 ） and low- dose Mog group （0.2 g·kg-1 ）. Except for normal group the other rats were sc 25% CC14 （2 g·kg-1 ） once every two week for eight weeks to establish chronic liver injury （the first time sc 25% CC14 5 mL ·kg-1 ）. The effects of Mog on the activities of ALT and AST, the content of hyaluronic acid （HA） , procollagen Ⅲ N-terminal petide （PIll NP）, hydroxyproline （Hyp） in serum, the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, the content of malondiadehyde （MDA） in liver tissue was observed. The effect of Mog on transforming growth factor β1 （TGF-β1） expression in liver tissue was observed and the histological changes of liver were detected. Result： Mog could inhibit the increasing in ALT and AST in serum of the acute hepatic injury induced by CC14 , and decrease the degree of the histological changes of liver. Mog could significantly decrease the elevation of serum ALT and AST activities, decrease the content of HA, PⅢ NP, Hyp in serum;
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第2期196-200,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西省自然科学基金项目(桂科自0229042)
广西省教育厅课题(桂教科研[2009]25号)
