摘要
目的分析不同类型Mg-1Zn-0.3Zr-2Gd-1Ca合金植入材料对大鼠骨组织的生物相容性,探讨其在大鼠骨质疏松性骨折固定中的可行性及安全性,评估其应用价值。方法设普通Mg-1Zn-0.3Zr-2Gd-1Ca合金组(A组)、壳聚糖涂层镁钙合金组(B组)、假手术组(C组) 3组大白鼠为研究对象,建模后予A、B组体内放置相应合金植入物。观察术后一般情况,分别于术后不同时间(7、14、21 d)分批处死大鼠并取出合金植入物,行扫描电镜观察了解术后合金降解情况,行血清学检测了解Mg-1Zn-0.3Zr-2Gd-1Ca合金对大鼠肝、肾功能的生物学毒性。结果①术后大鼠生命体征平稳,未出现手术相关并发症;②扫描电镜示,随术后时间的延长,A组植入物逐渐由点腐蚀变为面腐蚀,而B组试样表面仅见轻微腐蚀,结构完整,A组腐蚀速率明显高于B组;③手术前后各时间点,各组肝、肾功能变化差异均无统计学意义(P>0.05);各组血清镁离子、钙离子变化差异均无统计学意义(P>0.05)。结论Mg-1Zn-0.3Zr-2Gd-1Ca合金在大鼠体内生物相容性良好,且生物学毒性小、安全性高。壳聚糖涂层Mg-1Zn-0.3Zr-2Gd-1Ca合金体内降解速率低于普通Mg-1Zn-0.3Zr-2Gd-1Ca合金,具有一定的临床应用价值。
Objective To analyze the biocompatibility of different types of Mg-1Zn-0.3Zr-2Gd-1Ca alloy implants on bone tissue in rats,explore the feasibility and safety of fixation of osteoporotic fractures in rats for evaluating the application value. Methods The rats as research object were divided into normal Mg-1Zn-0.3Zr-2Gd-1Ca alloy group (group A),chitosan-coated Mg-1Zn-0.3Zr-2Gd-1Ca alloy group (group B) and sham operation group (group C). Corresponding alloy implants were placed in group A and B after modeling.The general condition was observed after operation,rats were sacrificed at different times (7,14,21 days)respectively,and the alloy implants were removed,the scanning electron microscopy was performed to observe the degradation of Mg-1 Zn-0. 3 Zr-2 Gd-1 Ca alloy in the operation area,and serological examination was performed to understand the biological toxicity of Mg-1 Zn-0. 3 Zr-2 Gd-1 Ca alloy on liver and kidney function in rats. Results ①Postoperative vital signs were stable and no operative complications occurred.②Scanning electron microscopy showed that the implants in group A gradually changed from pitting corrosion to surface corrosion with the prolongation of postoperative time,while the surface of group B was only slightly corroded and structural integrity,and the corrosion rate in group A was significantly higher than that in group B. ③ There was no significant difference in liver and kidney function at each time point before and after operation (P > 0. 05),and there was no significant difference in serum magnesium and calcium among groups (P >0.05). Conclusion Magnesium calcium alloy has good biocompatibility in rats,small biological toxicity and high safety. The degradation rate of Mg-1Zn-0.3Zr-2Gd-1Ca alloy coated with chitosan in vivo is lower than that of Mg-1Zn-0.3Zr-2Gd-1Ca alloy coated with chitosan,which has certain clinical value.
出处
《河南科技大学学报（医学版）》
2019年第1期1-5,共5页
Journal of Henan University of Science & Technology:Medical Science
基金
国家自然科学基金联合基金项目(U1404825)
河南省重点科技攻关项目(152102310012)
河南省自然科学基金项目(162300410241)
洛阳市科技发展计划项目(1503006A-3)
