摘要
从云南不同地区长春花 (Catharanthutsroseus)植株上分离到 4株能产长春新碱的真菌[1 ] ,从筛选出1株综合条件较好的菌株 97CG3 (尖孢镰刀菌Fusariumoxysporum) ,利用高效液相色谱分析法及通过单因子实验和多因子正交实验 ,基本上确定了其最佳培养条件。为今后应用微生物发酵法生产长春新碱提供依据。
Four isolates of indophtic fungi producing vincristine were isolated from Catharanthus roseus of different areas in Yunnan province and the best one(97CG3) was selected from the four isolates It is Fusarium oxysporum The best culture conditions have been preliminarily obtained by the results of HPLC and the test of uni-factor and the orthogonal test This study will provide basis for the future massively industrialized vincristine production
出处
《楚雄师范学院学报》
2002年第6期39-41,共3页
Journal of Chuxiong Normal University
基金
国家自然科学基金资助项目(3 9860 0 0 5 )
关键词
长春新碱
内生真菌
发酵条件
vincristine
endophtic fungi
culture condition
