摘要
目的:探讨乌司他丁对脓毒症大鼠神经功能的保护作用方法:46只SD大鼠随机分为空白对照组(Norm组,n=8)、假手术组(Sham组,n=8)和盲肠结扎穿孔组(CLP组,n=30),仅CLP组进行盲肠结扎穿孔术(cecal ligation and puncture,CLP)。24 h后评估各组的神经功能缺损评分(neurological deficient score,NDS),将脓毒症动物(S^+)随机分为S^+-control组和S^+-UTI组,检测Norm组、Sham组、S^+-control组和S^+-UTI组血清S100β的含量,随后腹腔注射乌司他丁(Ulinastatin,UTI)10万U/kg(tid,6 h/次)或等体积生理盐水。干预后24 h再次评估NDS,检测血清S100β的含量。结果:CLP术后24 h,约35%(10/29)的大鼠未发展为脓毒症;与Sham组相比,S^+的NDS明显降低(P<0.01),血清S100β明显升高(P<0.01)。干预后24 h,S^+-UTI组的NDS高于S^+-control组(P=0.019),而血清S100β低于S^+-control组(P=0.015)。结论:乌司他丁对脓毒症大鼠的神经功能具有保护作用。
Objective:To explore the effect of Ulinastatin on sepsis in a rat cecal ligation and puncture model.Methods:Forty-six healthy male Sprague-Dawley rats were randomly divided into Norm group(n=8),Sham group(n=8)and CLP group(n=30). Cecal ligation and puncture(CLP)was performed in CLP group. Septic animals(S^+)animals were randomized into S^+-control group and S^+-UTI group. Neurological deficient score(NDS)and levels of serum S100β were in vestigated in Norm,Sham,S^+-control and S^+-UTI group at 24 hours post the surgery,respectively. Ulinastatin(100,000 U/kg)or isometric normal saline was intraperitoneally administered every six hours for a total of three times. 24 hours later,animals were sacrificed for the detection of serum S100β after the investigation of NDS. Results:Approximately thirty-five percent of animals(10/29)didn′t progress to sepsis after the CLP insults. Significantly lower NDS and higher serum S100β were observed in S^+group when compared with Sham group at 24 hours after the surgery(both P< 0.01). At 24 hours after the administration of UTI,NDS was obviously higher(P<0.05),while serum S100β were statistically lower(P<0.05)in S^+-UTI group in comparison with S^+-control group. Conclusion:Ulinastatin protected neurological function in a validated septic model induced by cecal ligation and puncture.
出处
《岭南急诊医学杂志》
2017年第2期107-109,共3页
Lingnan Journal of Emergency Medicine
基金
天普研究基金项目(UF201328)
关键词
脓毒症
乌司他丁
神经功能
盲肠结扎穿孔法
sepsis
ulinastatin
neurological function
cecal ligation and puncture
