摘要
目的 检测胃癌常规病理检查阴性的淋巴结微转移的发生及与其它临床参考指标的关系。方法 利用逆转录—聚合酶链反应 (RT -PCR)方法检测 6 8枚胃癌胃周淋巴结癌胚抗原 (CEA)mRNA基因表达 ,同时比较RT -PCR与免疫组化 (IHC)方法的检测敏感性。结果 CEAmRNART -PCR是一种很敏感的方法 ,可以检测1/10 6个转移的癌细胞 ;检测 19例胃癌患者取材的 6 8枚胃周淋巴结 ,IHC阳性率 2 8% (19/6 8) ,RT -PCR阳性率5 7% (39- 6 8) ,两组之间差异有非常显著性意义 (P <0 .0 1) ;RT -PCR阳性率与胃癌临床参考指标密切相关 ,且随着病期进展而增大。结论 CEAmRNART -PCR是比免疫组化更敏感的方法 ,可以预测胃癌淋巴结微转移 。
Objectives To deection the emergence that lymph node that the result of the routine pathology of gastric cancer checks is negative micrometastases and its relation with other clinical index.Methods To utilize the method of reverse transcriptase-polymerase chain reaction(RT-PCR) to test 68 CEA mRNA's gene expression and compare the detection sensitiveness between RT-PCR and immunohistochemical staining(IHC).Results The method of CEA mRNA RT-PCR is higly sensitive,could detection 1/10 6 transformed cancer cell,detection 68 lymph node from 19 patient of gastric cancer.IHC positive rate is 28%(19/68),RT-PCR positive rate is 57%(39/68),and the difference between the two groups is very apparent(P<0.01);RT-PCR positive rate is closely related with clinical index,and increase as the disease develops.Conclusions CEA mRNA RT-PCR is more highly sensitive than IHC and can predict the lymph node micrometastases of gastric cancer.
出处
《宁夏医学杂志》
CAS
2003年第4期198-200,共3页
Ningxia Medical Journal
基金
宁夏自然科学基金资助课题 (编号 :2 0 0 1A30 0 3)
