摘要
目的 研究雷公藤多甙对肌肉本身收缩力的作用。方法 用电子刺激器刺激 3 0只大鼠膈神经 -膈肌标本的膈神经和直接刺激 45只蟾蜍腓肠肌标本的方法 ,研究雷公藤多甙对肌肉本身收缩力的影响。结果 在三分之一最适刺激强度时 ,雷公藤多甙在 2 0 mg/ L组 ,前 60 min使大鼠膈肌收缩幅度增大 ,此后至 90 min为止 ,收缩幅度无增大 ;在 40 mg/ L、60 mg/ L组中前 60 min未引起大鼠膈肌收缩幅度减小。在三分之一最适刺激强度直接刺激蟾蜍腓肠肌标本时 ,雷公藤多甙在 60 mg/ L组前 3 0 min未引起蟾蜍腓肠肌收缩张力减小。而溶剂二甲亚砜在三分之一最适刺激强度时使大鼠膈肌收缩幅度和蟾蜍腓肠肌收缩张力均明显减小。结论 雷公藤多甙对肌肉本身收缩力有一定增强作用 ;
Objective To investigate the direct effects of Tripterygium Wilfordii Polyglycosidium (TWP) on capability of muscle contraction. Methods Using electronic stimulator to stimulate the phrenic nerve of the isolated phrenic nerve diaphragm preparation of 30 rats or directly stimulate the isolated gastrocnemius muscle preparation of 45 toads in vitro, we studied the effects of TWP on capability of muscle intrinsic contraction. Results TWP in 20 mg/L increased the amplitude of muscle contraction in initial 60 min but did not make further increase of the amplitude of muscle contraction from 60 min to 90 min in the isolated phrenic nerve diaphragm preparation of rat under one third optimal stimulus. TWP in 40 mg/L and in 60 mg/L did not cause decrease of amplitude of muscle contraction in initial 60 min in isolated phrenic nerve diaphragm preparation of rat under one third optimal stimulus. TWP in 60 mg/L did not cause decrease of tension of signal contraction in initial 30 min in isolated gastrocnemius muscle preparation of toad under one third optimal direct stimulus. Solvent DMSO could obviously reduce the tension of muscle contraction both in isolated phrenic nerve diaphragm preparation of rat and in isolated gastrocnemius muscle preparation of toad under one third optimal stimulus. Conclusion TWP can limitedly enhance the capability of muscle contraction; Solvent DMSO can restrain muscle contraction.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2003年第2期289-291,294,共4页
Journal of Sichuan University(Medical Sciences)
基金
卫生部科研基金 (编号 98-2 -197)资助
关键词
雷公藤多甙
膈神经-膈肌
腓肠肌
肌肉收缩
TWP Isolated phrenic nerve diaphragm preparation Isolated gastrocnemius muscle preparation Muscle contraction
