摘要
用自行改良的氯化苄法,从液体培养的双歧杆菌菌体中提取基因组DNA,用紫外吸收光谱、琼脂糖凝胶电泳等方法进行鉴定。结果表明,该法提取的基因组DNA分子量高,收获量大,蛋白质污染少,而且快速、高效、经济和实用。以该基因组DNA为模板,用随机引物S256通过AP—PCR扩增出清晰的RAPD谱带,并呈现出一定程度的遗传多态性。讨论了RAPD指纹图谱在双歧杆菌分类鉴定及工业菌株分子标记中潜在的应用价值。
H igh molecular weight genomic DNA wa s extracted from Bifidobacteria sp using ben zy1chloride method modi fied by us.The properities of the DNAs were identified by U.V absorption rate,RNase digestion and agarose gel elec trophoesis.Using the DNA as template and S256as primer,RAPD fingerprinting was obtained.The potential application of RAPD fi ngerprinting in molecular identification and m olec ular typing of Bifidobacferium strains were discussed in this paper.The re sults showed that the modified method is rapid,efficient ,economic and safe,s uitable for extraction of genomic DNA from Bifidobacteria and other lactic acid bacteria.
出处
《中国乳品工业》
CAS
北大核心
2003年第2期3-5,共3页
China Dairy Industry
基金
江苏省高技术发展研究计划项目(BG2002322)
