摘要
用随机排列碱基顺序的寡核苷酸引物，对蚕、鱼、蛇、家猪等动物的基因组ＤＮＡ进行ＰＣＲ扩增，获得了这些动物的随机扩增多态ＤＮＡ指纹图谱．对影响扩增结果的几个因素进行了分析。
Species specific RAPD fingerprints of pig, snake, eel, and silkworm were generated by random amplified polymorphic DNA (RAPD). Some conditions of the RAPD reaction procdeure may influence the result. Polymerase, annealing temperature, and primer, are important factors in obtaining good DNA yields and optimal fragment patterns. RAPD has been proved to be an efficient method in genetic analysis of animals.
出处
《南京师大学报（自然科学版）》
CAS
CSCD
1996年第2期66-70,共5页
Journal of Nanjing Normal University(Natural Science Edition)
基金
南京师范大学和江苏省养猪行业协会合作项目
关键词
动物
DNA指纹图谱
聚合酶链反应
RAPD
animal, random amplified polymorphic DNA (RAPD), DNA fingerprints, PCR
