摘要
目的:探讨MUC5AC,MUC6核粘蛋白合成肽在诱导小鼠抗肿瘤免疫反应中的作用.方法:采用动物实验方法观察MUC5AC和MUC6黏核蛋白的合成肽诱导小鼠的细胞和体液免疫反应及其淋巴细胞体外抗肿瘤作用.结果:MUC5AC,MUC6合成肽可诱导小鼠产生相应的抗体及迟发型过敏反应,但不能产生脾淋巴细胞的明显增生及体外抗7901胃癌细胞株的作用.结论:MUC5AC,MUC6合成肽免疫小鼠可引发体液及细胞免疫反应,但尚不足以产生淋巴细胞毒作用.
AIM:To evaluate the immune response and anti-tumor activity induced by synthetic polypeptides of MUC5AC and MUC6 apomucins in mice. METHODS:The cellular and humoral immunity induced by MUC5AC and MUC6 synthetic polypeptides were examined in mouse,proliferation of lymphocyte and cytotoxicity of T lymphocytes were assessed by 3H-TDR incorporation assay. RESULTS:The conjugated MUC5AC and MUC6 synthetic polypeptides could induce B cells in mice to produce high titre antibodies against the immunized peptides and delayed- type hypersensitivity. But they could not induce significant proliferation of lymphocyte and specific cytotoxic T-lym- phocytes (CTL) in vitro against 7901 gastric cancer cells. This may be caused by the experimental design, inefficient epitope of synthetic polypeptides, or MHC restriction. CONCLUSION:MUC5AC-KLH and MUC6-KLH synthetic polypeptide conjugates can induce cellular and humoral immunity other than cytotoxic T lymphocytes of anti-tumor effect.
出处
《世界华人消化杂志》
CAS
2003年第3期314-317,共4页
World Chinese Journal of Digestology
